Stimulation of IL-6 Cytokines in Fibroblasts by Toll-like Receptors 2
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Undergraduate course
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Sage Publications Inc
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Article
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Abstract
The osteotropic interleukin-6 (IL-6) types of cytokines IL-6, IL-11, and leukemia inhibitory factor (LIF), but not oncostatin M, are expressed by human gingival fibroblasts, and their expressions are regulated by IL-1 and tumor necrosis factor-a (TNF-alpha). In the present study, we investigated whether signaling through Toll-like receptor 2 (TLR2) can affect the expression of these cytokines in human gingival fibroblasts. Lipopolysaccharide (LPS) from P. gingivalis was found to stimulate IL-6 and LIF mRNA and protein, but not IL-11 or OSM mRNA. Using two synthetic ligands acting specifically at TLR2 and siRNA knockdown of TLR2, we demonstrated the important role of TLR2 in the stimulation of IL-6 and LIF in gingival fibroblasts. Analysis of these data suggests that signaling through the innate immune system controls the expression of osteotropic cytokines in human gingival fibroblasts.
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Keywords
gingival fibroblasts, IL-6, LIF, IL-11, TLR
Language
English
Citation
Journal of Dental Research. Thousand Oaks: Sage Publications Inc, v. 89, n. 8, p. 802-807, 2010.
