Uso de implante 3D de copoliamida associada à elastômero termoplástico aliado ao enxerto autólogo de omento maior para reparação traqueal de coelhos (Oryctolagus cuniculus)
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2021-02-22
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Universidade Estadual Paulista (Unesp)
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Objetivos: Avaliar o uso da copoliamida associada à elastômero termoplástico (PCTPE) em próteses impressas em 3D para reparação de defeitos parciais da traqueia de coelhos associada ao enxerto autólogo de omento maior para auxiliar na regeneração tecidual. Metodologia: Foram utilizados 32 coelhos machos da raça Nova Zelândia Branco, submetidos à criação de um defeito parcial de traqueia na região ventral do 4º ao 8º anel traqueal. Os animais foram distribuídos em dois grupos com 16 animais cada: GI (grupo com implante) e GII (grupo com implante e enxerto autólogo de omento maior). Cada grupo foi subdividido (n=4) de acordo com o momento das eutanásias, programadas com intervalo de sete (GI7 e GII7), quinze (GI15 e GII15), trinta (GI30 e GII30) e sessenta dias (GI60 e GII60). Resultados: Um animal do GI60 veio a óbito. Até os 60 dias de avaliação sugere-se que não ocorreu a reepitelização da traqueia devido à presença de secreção intraluminal visibilizada por meio da traqueoscopia em ambos os grupos sem diferença significativa (p>0,05), entretanto existiram indicadores positivos da interação implante-traqueia como visibilização de pontos de sutura, presença de tecido recobrindo a prótese e alterações clínicas pouco presentes e importantes. Por estes motivos o PCTPE mostrou resultados promissores para reconstrução de defeitos parciais da traqueia em coelhos. O enxerto de omento não apresentou resultados satisfatórios no presente estudo, pois o GII apresentou complicações como exacerbação da inflamação possivelmente pela necrose e consequente perpetuação de infeção no local.
Objectives: Evaluating the use of copolyamide associated with thermoplastic elastomer (PCTPE) in 3D printed prostheses used to repair partial defects in the trachea of rabbits and comparing the tissue regeneration when associated with autologous greater omental graft. Methodology: 32 male New Zealand White rabbits were submitted to the induction of a partial defect in the ventral region from the 4th to the 8th tracheal rings. The animals were divided into two groups of 16: GI (group with implant) and GII (group with implant and greater omentum graft). Each group was subdivided (n=4) according to the time of euthanasia, scheduled with an interval of seven (GI7 and GII7), fifteen (GI15 and GII15), thirty (GI30 and GII30) and sixty days (GI60 and GII60). Results: One animal from GI60 has died. Up to the 60 days of evaluation, it was suggested that the tracheal reepithelization did not occur due to the presence of intraluminal secretion visualized by tracheoscopy in both groups without significant difference (p>0,05), however there were some positive indicators of the implant-trachea interaction visualized by the sutures, presence of tissue covering the prosthesis and little present and important clinical changes. For these reasons, the PCTPE showed promising results for the reconstruction of partial defects of the trachea in rabbits. The greater omentum graft has not present satisfactory results in the present study, because GII has presented complications such as exacerbation of inflammation, which is suggested due to the necrosis and the consequent perpetuation of infection at the site.
Objectives: Evaluating the use of copolyamide associated with thermoplastic elastomer (PCTPE) in 3D printed prostheses used to repair partial defects in the trachea of rabbits and comparing the tissue regeneration when associated with autologous greater omental graft. Methodology: 32 male New Zealand White rabbits were submitted to the induction of a partial defect in the ventral region from the 4th to the 8th tracheal rings. The animals were divided into two groups of 16: GI (group with implant) and GII (group with implant and greater omentum graft). Each group was subdivided (n=4) according to the time of euthanasia, scheduled with an interval of seven (GI7 and GII7), fifteen (GI15 and GII15), thirty (GI30 and GII30) and sixty days (GI60 and GII60). Results: One animal from GI60 has died. Up to the 60 days of evaluation, it was suggested that the tracheal reepithelization did not occur due to the presence of intraluminal secretion visualized by tracheoscopy in both groups without significant difference (p>0,05), however there were some positive indicators of the implant-trachea interaction visualized by the sutures, presence of tissue covering the prosthesis and little present and important clinical changes. For these reasons, the PCTPE showed promising results for the reconstruction of partial defects of the trachea in rabbits. The greater omentum graft has not present satisfactory results in the present study, because GII has presented complications such as exacerbation of inflammation, which is suggested due to the necrosis and the consequent perpetuation of infection at the site.
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Aloenxertos, Aparelho respiratório, Biomateriais, Prótese