Publication: Immunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors
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Date
2011-02-01
Advisor
Coadvisor
Graduate program
Undergraduate course
Journal Title
Journal ISSN
Volume Title
Publisher
Arquivo Brasileiro Medicina Veterinaria Zootecnia
Type
Article
Access right
Acesso aberto
Abstract
Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the gamma 2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 gamma 2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with gamma 2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 gamma 2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 gamma 2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results.
Description
Keywords
Language
English
Citation
Arquivo Brasileiro de Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 63, n. 1, p. 28-35, 2011.
