A Rheological Study of the Association and Dynamics of MUC5AC Gels
Author(s)
Rubinstein, Michael; Wagner, Caroline E; Turner, Bradley S.; McKinley, Gareth H; Ribbeck, Katharina
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The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micrometer-sized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully constituted mucus.
Date issued
2017-11Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical EngineeringJournal
Biomacromolecules
Publisher
American Chemical Society (ACS)
Citation
Wagner, Caroline E. et al. “A Rheological Study of the Association and Dynamics of MUC5AC Gels.” Biomacromolecules 18, 11 (October 2017): 3654–3664 © 2017 American Chemical Society
Version: Author's final manuscript
ISSN
1525-7797
1526-4602