Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/34099
Title: | FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices | Authors: | Lerner, Eitan Barth, Anders HENDRIX, Jelle Ambrose, Benjamin Birkedal, Victoria Blanchard, Scott C Börner, Richard Sung Chung, Hoi Cordes, Thorben Craggs, Timothy D Deniz, Ashok A Diao, Jiajie Fei, Jingyi Gonzalez, Ruben L Gopich, Irina V Ha, Taekjip Hanke, Christian A Haran, Gilad Hatzakis, Nikos S Hohng, Sungchul Hong, Seok-Cheol Hugel, Thorsten Ingargiola, Antonino Joo, Chirlmin Kapanidis, Achillefs N Kim, Harold D Laurence, Ted Lee, Nam Ki Lee, Tae-Hee Lemke, Edward A Margeat, Emmanuel Michaelis, Jens Michalet, Xavier Myong, Sua Nettels, Daniel Peulen, Thomas-Otavio Ploetz, Evelyn Razvag, Yair Robb, Nicole C Schuler, Benjamin Soleimaninejad, Hamid Tang, Chun Vafabakhsh, Reza Lamb, Don C Seidel, Claus Am Weiss, Shimon |
Issue Date: | 2021 | Publisher: | ELIFE SCIENCES PUBLICATIONS LTD | Source: | eLife, 10 (Art N° e60416) | Abstract: | Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB-Dev. This position paper describes the current 'state of the art' from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of 'soft recommendations' about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage 'open science' practices. | Keywords: | FRET;biochemistry;biomolecules;chemical biology;community;conformation;dynamics;molecular biophysics;single-molecule;structural biology | Document URI: | http://hdl.handle.net/1942/34099 | ISSN: | 2050-084X | e-ISSN: | 2050-084X | DOI: | 10.7554/eLife.60416 | ISI #: | WOS:000635522800001 | Rights: | This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available underthe Creative Commons CC0 public domain dedication. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
elife-60416-v3.pdf | Published version | 6.46 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.