The effect of inhibitors of plasma membrane H+-ATPase and oxidoreductases on NH+4uptake by Pisum arrense roots
Authors:
- Halina Lekacz,
- Waldemar Karcz,
- Genowefa Kubik-Dobosz,
- Aleksandra Turska,
- Józef Buczek
Abstract
The effect of inhibitors of plasma membrane oxidoreductases (quinacrine and dicumarol) and H+-ATPase (dicyclohexylcarbodiimide and orthovanadate) on ammonium uptake by Piswn arve11se seedlings and the activities of H+-ATPase and NADHferricyanide oxidoreductase was investigated. The uptake solution contained 50 µM NH4 +. In I h experiments, quinacrine and dicumarol depressed strongly and irreversibly the rate of NH4 + uptake and markedly inhibited the activity of NADH-ferricyanide oxidoreductase in the plasma membrane vesicles prepared from root cells. Simultaneously, sodium onhovanadate inhibited the activity of plasma membrane H+-ATPase increased the rate of NH/ uptake. Dicyclohexylcarbodiimide inhibited H+ -ATPase activity and increased efflux of NH4 + from roots to ambient solution. The results indicate on the lack of direct connection between uptake rate of 50 µM NH4 + and H+-ATPase activity, and suggest thai mem brane redox systems play a predominant role in this process.
- Record ID
- USLae17b6ac51c8493380a8de007ace8dcd
- Author
- Journal series
- Acta Societatis Botanicorum Poloniae, ISSN 0001-6977, e-ISSN 2083-9480
- Issue year
- 1994
- No
- 3
- Pages
- 287-292
- Publication size in sheets
- 0.30
- Keywords in English
- Ammonium, Uptake
- ASJC Classification
- DOI
- DOI:10.5586/asbp.1994.039 Opening in a new tab
- Handle.net URL
- hdl.handle.net/20.500.12128/15082 Opening in a new tab
- Language
- pol (pl) Polish
- File
-
- File: 1
- The effect of inhibitors of plasma membrane H+-ATPase and oxidoreductases on NH+4uptake by Pisum arrense roots, File Kubik-Dobosz_The_effect_of_inhibitors_of_plasma.pdf / 2 MB
- Kubik-Dobosz_The_effect_of_inhibitors_of_plasma.pdf
- publication date: 06-02-2024
- The effect of inhibitors of plasma membrane H+-ATPase and oxidoreductases on NH+4uptake by Pisum arrense roots, File Kubik-Dobosz_The_effect_of_inhibitors_of_plasma.pdf / 2 MB
-
- Score (nominal)
- 0
- Score source
- journalList
- Publication indicators
- Uniform Resource Identifier
- https://opus.us.edu.pl/info/article/USLae17b6ac51c8493380a8de007ace8dcd/
- URN
urn:uni-kat-prod:USLae17b6ac51c8493380a8de007ace8dcd
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.