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Unravelling the nanometre-scale stimuli-responsive properties of natural rubber latex particles using atomic force microscopy

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Gaboriaud, F. [Michelin] De Gaudemaris, B. [Michelin] Rousseau, T. [Michelin] Derclaye, Sylvie [UCL] Dufrêne, Yves [UCL]

Natural rubber (NR) latex particles exhibit a core-shell structure, with a polyisoprene elastomer as the core and a thin layer made of proteins and lipids as the shell. Knowledge of the surface properties and interactions of core-shell NR particles is a key to gain a detailed understanding of their colloidal behaviour and to efficiently exploit them in industrial processes. Here, we report the first nanoscale measurement of the stimuli-responsive behaviour of single NR latex particles by atomic force microscopy (AFM), revealing that their molecular interactions are tightly controlled by salt conditions. High-resolution imaging, combined with protease treatments, confirmed the presence of a soft layer of proteins on the surface of the NR particles. Upon approach of the particles in diluted solutions of monovalent salts (1 mM NaCl), the AFM tip experienced a long-range repulsive force, reflecting electrostatic surface forces and compression of the soft, loosely structured protein layer. Upon retraction, sharp adhesion events were observed due to strong attractive interactions between the tip and proteins. When increasing the ionic strength (100 mM NaCl) or ion valency (1 mM and 100 mM MgCl 2), the long-range repulsion was no longer seen, while nanotubes made of proteins and/or lipids were found to strengthen the tip-particle adhesive bonds. These findings, which correlate with the macroscopic aggregation of the particles, indicate that high ionic strength or ion valency leads to more compact shells by lowering repulsive intermolecular forces. The nanoscale measurements presented here provide new opportunities for quantifying the stimuli-responsive properties of soft colloids, like NR particles, and for tuning their interfacial interactions for industrial applications. © 2012 The Royal Society of Chemistry.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "Soft Matter" - Vol. 8, no. 9, p. 2724-2729 (2012)
Peer reviewed yes
Publisher Royal Society of Chemistry
issn 1744-683X
e-issn 1744-6848
Publication status Publié
Affiliation UCL - SST/IMCN/BSMA - Bio and soft matter
Keywords Adhesive bond ; AFM tip ; Intermolecular forces ; Latex particles ; Long-range repulsions ; Monovalent salt ; Nano-scale measurements ; Natural rubber latex ; Natural rubbers ; NR latex ; Protease treatment ; Protein layers ; Core shell structure ; Repulsive forces ; Soft colloids ; Soft layers ; Stimuli-responsive ; Stimuli-responsive properties ; Thin layers ; Valencies ; Adhesion ; Atomic force microscopy ; Chemical bonds ; Core-shell ; Industrial applications ; Ionic strength ; Latexes ; Lipids ; Nanotechnology ; Nanotubes ; Proteins ; Rubber ; Shells (structures) ; Sodium chloride ; Diluted solutions ; Surface properties ; Agglomeration ; Electrostatic surfaces ; High ionic strength ; High-resolution imaging ; Industrial processs ; Interfacial interaction
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Bibliographic reference Gaboriaud, F. ; De Gaudemaris, B. ; Rousseau, T. ; Derclaye, Sylvie ; Dufrêne, Yves. Unravelling the nanometre-scale stimuli-responsive properties of natural rubber latex particles using atomic force microscopy. In: Soft Matter, Vol. 8, no. 9, p. 2724-2729 (2012)
Permanent URL http://hdl.handle.net/2078.1/111425