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Effect of 2,4,6-trinitrotoluene on soil bacterial communities.

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George, Isabelle [UCL] Eyers, Laurent [UCL] Stenuit, Benoît [UCL] Agathos, Spiros N. [UCL]

To gain insight into the impact of 2,4,6-trinitrotoluene (TNT) on soil microbial communities, we characterized the bacterial community of several TNT-contaminated soils from two sites with different histories of contamination and concentrations of TNT. The amount of extracted DNA, the total cell counts and the number of CFU were lower in the TNT-contaminated soils. Analysis of soil bacterial diversity by DGGE showed a predominance of Pseudomonadaceae and Xanthomonadaceae in the TNT-contaminated soils, as well as the presence of Caulobacteraceae. CFU from TNT-contaminated soils were identified as Pseudomonadaceae, and, to a lesser extent, Caulobacteraceae. Finally, a pristine soil was spiked with different concentrations of TNT and the soil microcosms were incubated for 4 months. The amount of extracted DNA decreased in the microcosms with a high TNT concentration [1.4 and 28.5 g TNT/kg (dry wt) of soil] over the incubation period. After 7 days of incubation of these soil microcosms, there was already a clear shift of their original flora towards a community dominated by Pseudomonadaceae, Xanthomonadaceae, Comamonadaceae and Caulobacteraceae. These results indicate that TNT affects soil bacterial diversity by selecting a narrow range of bacterial species that belong mostly to Pseudomonadaceae and Xanthomonadaceae.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2008
Journal information "Journal of Industrial Microbiology and Biotechnology" - Vol. 35, no. 4, p. 225-236 (2008)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 1367-5435
e-issn 1476-5535
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Trinitrotoluene - metabolism - pharmacology ; Stem Cells ; Soil Pollutants - metabolism - pharmacology ; Soil Microbiology ; Soil - analysis ; Sequence Analysis, DNA ; RNA, Ribosomal, 16S - analysis - genetics ; Phylogeny ; Molecular Sequence Data ; DNA, Ribosomal - analysis - genetics ; DNA, Bacterial - analysis - genetics ; DNA Fingerprinting ; Biodegradation, Environmental ; Bacteria - classification - drug effects - genetics - metabolism
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Bibliographic reference George, Isabelle ; Eyers, Laurent ; Stenuit, Benoît ; Agathos, Spiros N.. Effect of 2,4,6-trinitrotoluene on soil bacterial communities.. In: Journal of Industrial Microbiology and Biotechnology, Vol. 35, no. 4, p. 225-236 (2008)
Permanent URL http://hdl.handle.net/2078.1/11264