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Cold exposure and associated metabolic changes in adult tropical beetles exposed to fluctuating thermal regimes

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Lalouette, Lisa [Université de Rennes] Koštál , Vladimir [Institute of Entomology, Biology Centre, Ceske Budejovice, Czech Republic] Colinet, Hervé [UCL] Gagneul, David [Université de Rennes] Renault, David [Université de Rennes]

Environmental stress deleteriously affects every aspect of an ectotherm’s biological function. Frequent exposure of terrestrial insects to temperature variation has thus led to the evolution of protective biochemical and physiological mechanisms. However, the physiological mechanisms underlying the positive impact of fluctuating thermal regimes (FTRs) on the fitness and survival of cold-exposed insects have not been studied. We have thus investigated the metabolic changes in adults of the beetle Alphitobius diaperinus in order to determine whether FTRs trigger the initiation of a metabolic response involving synthesis of protective compounds, such as free amino acids (FAAs) and polyols. The metabolic profile was analyzed during constant fluctuating thermal regimes (the beetles had daily pulses at higher temperatures that enabled them to recover) and compared with constant cold exposure and untreated controls. The increase of several essential amino acids (Lys, Iso, Leu, Phe and Trp) in cold-exposed beetles supports the conclusion that it results from the breakdown of proteins. Some FAAs have been shown to have cryoprotective properties in insects, but the relationship between FAAs, cold tolerance and survival has not yet been well defined. Instead of considering FAAs only as a part of the osmo- and cryo- protective arsenal, they should also be regarded as main factors involved in the multiple regulatory pathways activated during cold acclimation. Under FTRs, polyol accumulation probably contributes to the increased duration of survival in A. diaperinus.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2007
Language Anglais
Journal information "FEBS Journal" - Vol. 274, no. 7, p. 1759–1767 (2007)
Peer reviewed yes
Publisher Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies
e-issn 1742-4658
Publication status Publié
Affiliation UCL - SC/BIOL - Département de biologie
Keywords amino acid ; fluctuating thermal regime ; insect ; polyol ; sugar
Links
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Bibliographic reference Lalouette, Lisa ; Koštál , Vladimir ; Colinet, Hervé ; Gagneul, David ; Renault, David. Cold exposure and associated metabolic changes in adult tropical beetles exposed to fluctuating thermal regimes. In: FEBS Journal, Vol. 274, no. 7, p. 1759–1767 (2007)
Permanent URL http://hdl.handle.net/2078.1/121726