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Stem cell bioprocess engineering towards cGMP production and clinical applications

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Sart, Sébastien [UCL] Schneider, Yves-Jacques [UCL] Li, Yan [Florida State University] Agathos, Spiros N. [UCL]

Stem cells, including mesenchymal stem cells and pluripotent stem cells, are becoming an indispensable tool for various biomedical applications including drug discovery, disease modeling, and tissue engineering. Bioprocess engineering, targeting large scale production, provides a platform to generate a controlled microenvironment that could potentially recreate the stem cell niche to promote stem cell proliferation or lineage-specific differentiation. This survey aims at defining the characteristics of stem cell populations currently in use and the present-day limits in their applications for therapeutic purposes. Furthermore, a bioprocess engineering strategy based on bioreactors and 3-D cultures is discussed in order to achieve the improved stem cell yield, function, and safety required for production under current good manufacturing practices

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2014
Language Anglais
Journal information "Cytotechnology : international journal of cell culture and biotechnology" - Vol. 66, no. 5, p. 709-722 (2014)
Peer reviewed yes
Publisher Springer Netherlands ((Netherlands) Dordrecht)
issn 0920-9069
e-issn 1573-0778
Publication status Publié
Affiliations UCL - SST/ELI/ELIM - Applied Microbiology
UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
MESH Subject Biotechnologie
Keywords Bioprocess engineering ; Bioreactor ; Differentiation ; Safety ; Stem cells
Links
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Bibliographic reference Sart, Sébastien ; Schneider, Yves-Jacques ; Li, Yan ; Agathos, Spiros N.. Stem cell bioprocess engineering towards cGMP production and clinical applications. In: Cytotechnology : international journal of cell culture and biotechnology, Vol. 66, no. 5, p. 709-722 (2014)
Permanent URL http://hdl.handle.net/2078.1/146299