User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

A prospective clinical study of ¹⁸F-FAZA PET-CT hypoxia imaging in head and neck squamous cell carcinoma before and during radiation therapy

Primary tabs

Servagi Vernat, Stéphanie [UCL] Differding, Sarah [UCL] Hanin, François-Xavier [UCL] Labar, Daniel [UCL] Bol, Anne [UCL] Lee, John Aldo [UCL] Grégoire, Vincent [UCL]

PURPOSE: Hypoxia in head and neck squamous cell carcinoma (HNSCC) is associated with poor prognosis and outcome. (18) F-Fluoroazomycin arabinoside (FAZA) is a positron emission tomography (PET) tracer developed to enable identification of hypoxic regions within tumor. The aim of this study was to evaluate the use of (18) F-FAZA-PET for assessment of hypoxia before and during radiation therapy. METHODS: Twelve patients with locally advanced HNSCC underwent (18) F-FAZA-PET scans before and at fraction 7 and 17 of concomitant chemo-radiotherapy. A hypoxic voxel was defined as a voxel expressing a standardized uptake value (SUV) equal or above the SUVmean of the posterior contralateral neck muscles plus three standard deviations. The fractional hypoxic volume fraction (FHV) and the spatial move of hypoxic volumes during treatment were analyzed. RESULTS: A hypoxic volume could be identified in ten patients before treatment. FAZA-PET FHV varied from 0 to 54.3% and from 0 to 41.4% in the primary tumor and in the involved node, respectively. Six out of these ten patients completed all the FAZA-PET-computed tomography (CT) during the radiotherapy. In all patients, FHV and SUVmax values decreased. All patient presented a spatial move of hypoxic volume, but only three patients had newborn hypoxic voxels after 17 fractions. CONCLUSION: This study indicated that (18) F-FAZA-PET could be used to identify and quantify tumor hypoxia before and during concomitant radio-chemotherapy in patients with locally advanced HNSCC. In addition to the information on prognostic value, the use of (18) F-FAZA-PET allowed the delineation of hypoxic volumes for dose escalation protocols. However, due to fluctuation of hypoxia during treatment, repeated scan will have to be performed (i.e. adaptive radiotherapy).

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2014
Language Anglais
Journal information "European Journal of Nuclear Medicine and Molecular Imaging" - Vol. 41, no. 8, p. 1544-1552 (2014)
Peer reviewed yes
Publisher Springer-Verlag Berlin ((Germany) Berlin)
issn 1619-7070
e-issn 1619-7089
Publication status Publié
Affiliations UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie
UCL - (SLuc) Service de médecine nucléaire
UCL - (SLuc) Service de radiothérapie oncologique
UCL - (SLuc) Service d'oto-rhino-laryngologie
UCL - (SLuc) Centre du cancer
Links
  1. VAUPEL P, Oxygenation status of malignant tumors: Pathogenesis of hypoxia and significance for tumor therapy, 10.1016/s0093-7754(01)90210-6
  2. Koukourakis Michael I., Bentzen Søren M., Giatromanolaki Alexandra, Wilson George D., Daley Frances M., Saunders Michele I., Dische Stanley, Sivridis Efthimios, Harris Adrian L., Endogenous Markers of Two Separate Hypoxia Response Pathways (hypoxia inducible factor 2 alpha and carbonic anhydrase 9) Are Associated With Radiotherapy Failure in Head and Neck Cancer Patients Recruited in the CHART Randomized Trial, 10.1200/jco.2005.02.7474
  3. Nordsmark Marianne, Bentzen Søren M., Rudat Volker, Brizel David, Lartigau Eric, Stadler Peter, Becker Axel, Adam Markus, Molls Michael, Dunst Juergen, Terris David J., Overgaard Jens, Prognostic value of tumor oxygenation in 397 head and neck tumors after primary radiation therapy. An international multi-center study, 10.1016/j.radonc.2005.06.038
  4. Fyles Anthony W, Milosevic Michael, Wong Raimond, Kavanagh Mary-Claire, Pintilie Melania, Sun Alex, Chapman William, Levin Wilf, Manchul Lee, Keane Thomas J, Hill Richard P, Oxygenation predicts radiation response and survival in patients with cervix cancer, 10.1016/s0167-8140(98)00044-9
  5. Lee Ding-Jen, Pajak Thomas F., Stetz Joanne, Order Stanley E., Weissberg Joseph B., Fischer James J., A phase I/II study of the hypoxic cell sensitizer misonidazole as an adjunct to high fractional dose radiotherapy in patients with unresectable squamous cell carcinoma of the head and neck: A RTOG randomized study (#79-04), 10.1016/0360-3016(89)90343-x
  6. Villar Alfonso, Martinez Jose Carlos, de Serdio Jose Luis, Chemoradiation for Advanced Head and Neck Cancer: Potential for Improving Results to Match Those of Current Treatment Modalities for Early-Stage Tumors—Long-Term Results of Hyperfractionated Chemoradiation With Carbogen Breathing and Anemia Correction With Erythropoietin, 10.1016/j.ijrobp.2007.08.068
  7. Overgaard Jens, Horsman Michael R., Modification of hypoxia-induced radioresistance in tumors by the use of oxygen and sensitizers, 10.1016/s1053-4296(96)80032-4
  8. Mizoe Jun-etsu, Hasegawa Azusa, Jingu Keiichi, Takagi Ryo, Bessyo Hiroki, Morikawa Takamichi, Tonoki Morio, Tsuji Hiroshi, Kamada Tadashi, Tsujii Hirohiko, Okamoto Yoshitaka, Results of carbon ion radiotherapy for head and neck cancer, 10.1016/j.radonc.2011.12.013
  9. Janssens Geert O., Rademakers Saskia E., Terhaard Chris H., Doornaert Patricia A., Bijl Hendrik P., van den Ende Piet, Chin Alim, Marres Henri A., de Bree Remco, van der Kogel Albert J., Hoogsteen Ilse J., Bussink Johannes, Span Paul N., Kaanders Johannes H., Accelerated Radiotherapy With Carbogen and Nicotinamide for Laryngeal Cancer: Results of a Phase III Randomized Trial, 10.1200/jco.2011.35.9315
  10. Toustrup Kasper, Sørensen Brita S., Alsner Jan, Overgaard Jens, Hypoxia Gene Expression Signatures as Prognostic and Predictive Markers in Head and Neck Radiotherapy, 10.1016/j.semradonc.2011.12.006
  11. Mueller-Klieser W., Schlenger K.-H., Walenta S., Gross M., Karbach U., Hoeckel M., Vaupel P., Pathophysiological approaches to identifying tumor hypoxia in patients, 10.1016/0167-8140(91)90182-g
  12. Busk Morten, Horsman Michael R., Jakobsen Steen, Keiding Susanne, van der Kogel Albert J., Bussink Johan, Overgaard Jens, Imaging Hypoxia in Xenografted and Murine Tumors With 18F-Fluoroazomycin Arabinoside: A Comparative Study Involving microPET, Autoradiography, Po2-Polarography, and Fluorescence Microscopy, 10.1016/j.ijrobp.2007.11.034
  13. Rajendran J.G., Hendrickson K.R.G., Spence A.M., Muzi M., Krohn K.A., Mankoff D.A., Hypoxia imaging-directed radiation treatment planning, 10.1007/s00259-006-0135-1
  14. Vaupel Peter, Mayer Arnulf, Hypoxia in cancer: significance and impact on clinical outcome, 10.1007/s10555-007-9055-1
  15. Lapi Suzanne E., Voller Thomas F., Welch Michael J., PET Imaging of Hypoxia, 10.1016/j.cpet.2009.05.009
  16. Lehtiö Kaisa, Oikonen Vesa, Nyman Samuel, Grönroos Tove, Roivainen Anne, Eskola Olli, Minn Heikki, Quantifying tumour hypoxia with fluorine-18 fluoroerythronitroimidazole ([ 18 F]FETNIM) and PET using the tumour to plasma ratio, 10.1007/s00259-002-1016-x
  17. Mahy Pierre, De Bast Marc, de Groot Tjibbe, Cheguillaume Arnaud, Gillart Jacques, Haustermans Karin, Labar Daniel, Grégoire Vincent, Comparative pharmacokinetics, biodistribution, metabolism and hypoxia-dependent uptake of [18F]-EF3 and [18F]-MISO in rodent tumor models, 10.1016/j.radonc.2008.06.008
  18. Mahy P., De Bast M., Gillart J., Labar D., Grégoire V., Detection of tumour hypoxia: comparison between EF5 adducts and [18F]EF3 uptake on an individual mouse tumour basis, 10.1007/s00259-005-0049-3
  19. Mahy P., De Bast M., Leveque P.H., Gillart J., Labar D., Marchand J., Gregoire V., Preclinical validation of the hypoxia tracer 2-(2-nitroimidazol-1-yl)-N-(3,3,3-[18F]trifluoropropyl)acetamide, [18F]EF3, 10.1007/s00259-004-1573-2
  20. Piert M, Machulla HJ, Picchio M, Reischl G, Ziegler S, Kumar P, et al. Hypoxia-specific tumor imaging with 18 F-fluoroazomycin arabinoside. J Nucl Med. 2005;46(1):106–13.
  21. Komar G., Seppanen M., Eskola O., Lindholm P., Gronroos T. J., Forsback S., Sipila H., Evans S. M., Solin O., Minn H., 18F-EF5: A New PET Tracer for Imaging Hypoxia in Head and Neck Cancer, 10.2967/jnumed.108.053785
  22. Chen Limin, Zhang Zhengwei, Kolb Hartmuth C., Walsh Joseph C., Zhang James, Guan Yihui, 18F-HX4 hypoxia imaging with PET/CT in head and neck cancer : a comparison with 18F-FMISO, 10.1097/mnm.0b013e3283571016
  23. Wijsman R, Kaanders JH, Oyen WJ, Bussink J. Hypoxia and tumor metabolism in radiation oncology: targets visualized by positron emission tomography. Q J Nucl Med Mol Imaging. 2013;57(3):244–56.
  24. Clausen Malene M, Hansen Anders E, af Rosenschold Per, Kjær Andreas, Kristensen Annemarie T, McEvoy Fintan J, Engelholm Svend A, Dose escalation to high-risk sub-volumes based on non-invasive imaging of hypoxia and glycolytic activity in canine solid tumors: a feasibility study, 10.1186/1748-717x-8-262
  25. Geets X, Gregoire V, Lee JA. Implementation of hypoxia PET imaging in radiation therapy planning. Q J Nucl Med Mol Imaging. 2013;57(3):271–82.
  26. Bentzen Søren M., Gregoire Vincent, Molecular Imaging–Based Dose Painting: A Novel Paradigm for Radiation Therapy Prescription, 10.1016/j.semradonc.2010.10.001
  27. Calais Gilles, Alfonsi Marc, Bardet Etienne, Sire Christian, Germain Thierry, Bergerot Philippe, Rhein Béatrix, Tortochaux Jacques, Oudinot Patrick, Bertrand Philippe, Randomized Trial of Radiation Therapy Versus Concomitant Chemotherapy and Radiation Therapy for Advanced-Stage Oropharynx Carcinoma, 10.1093/jnci/91.24.2081
  28. Bonner James A., Harari Paul M., Giralt Jordi, Azarnia Nozar, Shin Dong M., Cohen Roger B., Jones Christopher U., Sur Ranjan, Raben David, Jassem Jacek, Ove Roger, Kies Merrill S., Baselga Jose, Youssoufian Hagop, Amellal Nadia, Rowinsky Eric K., Ang K. Kian, Radiotherapy plus Cetuximab for Squamous-Cell Carcinoma of the Head and Neck, 10.1056/nejmoa053422
  29. EudraLex TRGMPitEU. Good Manufacturing Practice Medicinal Products for Human and Veterinary Use. Volume 4; Annex 3 Manufacture of Radiopharmaceuticals.
  30. Reischl G., Ehrlichmann W., Bieg C., Solbach C., Kumar P., Wiebe L.I., Machulla H.-J., Preparation of the hypoxia imaging PET tracer [18F]FAZA: reaction parameters and automation, 10.1016/j.apradiso.2004.12.004
  31. Souvatzoglou M., Grosu A. L., Röper B., Krause B. J., Beck R., Reischl G., Picchio M., Machulla H.-J., Wester H.-J., Piert M., Tumour hypoxia imaging with [18F]FAZA PET in head and neck cancer patients: a pilot study, 10.1007/s00259-007-0424-3
  32. Trinkaus Mateya E, Blum Rob, Rischin Danny, Callahan Jason, Bressel Mathias, Segard Tatiana, Roselt Peter, Eu Peter, Binns David, MacManus Michael P, Ball David, Hicks Rodney J, Imaging of hypoxia with18F-FAZA PET in patients with locally advanced non-small cell lung cancer treated with definitive chemoradiotherapy : 18F-FAZA PET in locally advanced NSCLC, 10.1111/1754-9485.12086
  33. Busk M., Mortensen L. S., Nordsmark M., Overgaard J., Jakobsen S., Hansen K. V., Theil J., Kallehauge J. F., D’Andrea F. P., Steiniche T., Horsman M. R., PET hypoxia imaging with FAZA: reproducibility at baseline and during fractionated radiotherapy in tumour-bearing mice, 10.1007/s00259-012-2258-x
  34. Mortensen Lise Saksø, Johansen Jørgen, Kallehauge Jesper, Primdahl Hanne, Busk Morten, Lassen Pernille, Alsner Jan, Sørensen Brita S., Toustrup Kasper, Jakobsen Steen, Petersen Jørgen, Petersen Henrik, Theil Jørn, Nordsmark Marianne, Overgaard Jens, FAZA PET/CT hypoxia imaging in patients with squamous cell carcinoma of the head and neck treated with radiotherapy: Results from the DAHANCA 24 trial, 10.1016/j.radonc.2012.09.015
  35. Grosu Anca-Ligia, Souvatzoglou Michael, Röper Barbara, Dobritz Martin, Wiedenmann Nicole, Jacob Vesna, Wester Hans-Jürgen, Reischl Gerald, Machulla Hans-Juergen, Schwaiger Markus, Molls Michael, Piert Morand, Hypoxia Imaging With FAZA-PET and Theoretical Considerations With Regard to Dose Painting for Individualization of Radiotherapy in Patients With Head and Neck Cancer, 10.1016/j.ijrobp.2007.05.079
  36. Mahy Pierre, De Bast Marc, Gallez Bernard, Gueulette John, Koch Cameron J, Scalliet Pierre, Grégoire Vincent, In vivo colocalization of 2-nitroimidazole EF5 fluorescence intensity and electron paramagnetic resonance oximetry in mouse tumors, 10.1016/s0167-8140(03)00028-8
  37. Tran Ly-Binh-An, Bol Anne, Labar Daniel, Jordan Bénédicte, Magat Julie, Mignion Lionel, Grégoire Vincent, Gallez Bernard, Hypoxia imaging with the nitroimidazole 18F-FAZA PET tracer: A comparison with OxyLite, EPR oximetry and 19F-MRI relaxometry, 10.1016/j.radonc.2012.04.011
  38. Gagel Bernd, Piroth Marc, Pinkawa Michael, Reinartz Patrick, Zimny Michael, Kaiser Hans J, Stanzel Sven, Asadpour Branka, Demirel Cengiz, Hamacher Kurt, Coenen Heinz H, Scholbach Thomas, Maneschi Payam, DiMartino Ercole, Eble Michael J, pO polarography, contrast enhanced color duplex sonography (CDS), [18F] fluoromisonidazole and [18F] fluorodeoxyglucose positron emission tomography: validated methods for the evaluation of therapy-relevant tumor oxygenation or only bricks in the puzzle of tumor hypoxia?, 10.1186/1471-2407-7-113
  39. Gagel Bernd, Reinartz Patrick, DiMartino Ercole, Zimny Michael, Pinkawa Michael, Maneschi Payam, Stanzel Sven, Hamacher Kurt, Coenen Heinz H., Westhofen Michael, Büll Ulrich, Eble Michael J., pO2 Polarography Versus Positron Emission Tomography ([18F] Fluoromisonidazole, [18F]-2-Fluoro-2’-Deoxyglucose) : An Appraisal of Radiotherapeutically Relevant Hypoxia, 10.1007/s00066-004-1229-y
  40. Bartlett R. M., Beattie B. J., Naryanan M., Georgi J.-C., Chen Q., Carlin S. D., Roble G., Zanzonico P. B., Gonen M., O'Donoghue J., Fischer A., Humm J. L., Image-Guided PO2 Probe Measurements Correlated with Parametric Images Derived from 18F-Fluoromisonidazole Small-Animal PET Data in Rats, 10.2967/jnumed.112.103523
  41. Zimny Michael, Gagel Bernd, DiMartino Ercole, Hamacher Kurt, Coenen Heinz H., Westhofen Martin, Eble Michael, Buell Ulrich, Reinartz Patrick, FDG—a marker of tumour hypoxia? A comparison with [18F]fluoromisonidazole and pO2-polarography in metastatic head and neck cancer, 10.1007/s00259-006-0175-6
  42. Nordsmark Marianne, Overgaard Marie, Overgaard Jens, Pretreatment oxygenation predicts radiation response in advanced squamous cell carcinoma of the head and neck, 10.1016/s0167-8140(96)91811-3
  43. Thorwarth Daniela, Eschmann Susanne-Martina, Scheiderbauer Jutta, Paulsen Frank, Alber Markus, Kinetic analysis of dynamic 18F-fluoromisonidazole PET correlates with radiation treatment outcome in head-and-neck cancer, 10.1186/1471-2407-5-152
  44. Rischin Danny, Fisher Richard, Peters Lester, Corry June, Hicks Rodney, Hypoxia in Head and Neck Cancer: Studies With Hypoxic Positron Emission Tomography Imaging and Hypoxic Cytotoxins, 10.1016/j.ijrobp.2007.05.043
  45. Duprez Fréderic, De Neve Wilfried, De Gersem Werner, Coghe Marc, Madani Indira, Adaptive Dose Painting by Numbers for Head-and-Neck Cancer, 10.1016/j.ijrobp.2010.03.028
  46. Madani Indira, Duprez Fréderic, Boterberg Tom, Van de Wiele Christophe, Bonte Katrien, Deron Philippe, Gersem Werner De, Coghe Marc, Neve Wilfried De, Maximum tolerated dose in a phase I trial on adaptive dose painting by numbers for head and neck cancer, 10.1016/j.radonc.2011.06.020
  47. Madani Indira, Duthoy Wim, Derie Cristina, De Gersem Werner, Boterberg Tom, Saerens Micky, Jacobs Filip, Grégoire Vincent, Lonneux Max, Vakaet Luc, Vanderstraeten Barbara, Bauters Wouter, Bonte Katrien, Thierens Hubert, De Neve Wilfried, Positron Emission Tomography-Guided, Focal-Dose Escalation Using Intensity-Modulated Radiotherapy for Head and Neck Cancer, 10.1016/j.ijrobp.2006.12.070
  48. Malinen Eirik, Søvik Åste, Hristov Dimitre, Bruland Øyvind S, Olsen Dag Rune, Adapting radiotherapy to hypoxic tumours, 10.1088/0031-9155/51/19/012
  49. Søvik Åste, Malinen Eirik, Skogmo Hege K., Bentzen Søren M., Bruland Øyvind S., Olsen Dag Rune, Radiotherapy Adapted to Spatial and Temporal Variability in Tumor Hypoxia, 10.1016/j.ijrobp.2007.04.027
  50. Thorwarth Daniela, Eschmann Susanne-Martina, Paulsen Frank, Alber Markus, Hypoxia Dose Painting by Numbers: A Planning Study, 10.1016/j.ijrobp.2006.11.061
Bibliographic reference Servagi Vernat, Stéphanie ; Differding, Sarah ; Hanin, François-Xavier ; Labar, Daniel ; Bol, Anne ; et. al. A prospective clinical study of ¹⁸F-FAZA PET-CT hypoxia imaging in head and neck squamous cell carcinoma before and during radiation therapy. In: European Journal of Nuclear Medicine and Molecular Imaging, Vol. 41, no. 8, p. 1544-1552 (2014)
Permanent URL http://hdl.handle.net/2078.1/153839