De Jaeger, Nathalie
[UCL]
De La Providencia Sanchez, Ivan Enriquez
[UCL]
Rouhier, H.
[UCL]
Declerck, Stephan
[UCL]
Aims: This study was performed to explore the compatibility and applicability of plant beneficial micro-organisms (i.e. Trichoderma harzianum MUCL 29707 and Glomus sp. MUCL 41833) co-entrapped in alginate beads. Methods and Results: Spores of Glomus sp. and conidia of T. harzianum were immobilized in alginate beads and the impacts of the saprotrophic fungi on the presymbiotic and symbiotic phase of the arbuscular mycorrhizal (AM) fungi evaluated under strict in vitro culture conditions. Our results demonstrated the capacity of both micro-organisms in combination to regrowth outside the calcium alginate coating. The presence of T. harzianum did not hinder the AM fungal development but rather stimulated its spore production and fitness. Conclusions: The combination of T. harzianum MUCL 29707 with Glomus sp. MUCL 41833 in alginate beads may represent a reliable alternative inoculum formulation for application in sustainable agriculture. Significance and Impact of the Study: The entrapment in the alginate beads of two fungi (i.e. a saprotroph and a symbiont) having beneficial effects on plants represents a promising formulation for the development of inoculants adapted to field application.
- Bashan Yoav, Inoculants of plant growth-promoting bacteria for use in agriculture, 10.1016/s0734-9750(98)00003-2
- Calvet C., Pera J., Barea J. M., Growth response of marigold (Tagetes erecta L.) to inoculation withGlomus mosseae, Trichoderma aureoviride andPythium ultimum in a peat-perlite mixture, 10.1007/bf02185378
- Chandanie W.A., Kubota M., Hyakumachi M., Interactions between the arbuscular mycorrhizal fungus Glomus mosseae and plant growth-promoting fungi and their significance for enhancing plant growth and suppressing damping-off of cucumber (Cucumis sativus L.), 10.1016/j.apsoil.2008.12.006
- Cranenbrouck Sylvie, Voets Liesbeth, Bivort Céline, Renard Laurent, Strullu Désiré-Georges, Declerck Stéphane, Methodologies for in Vitro Cultivation of Arbuscular Mycorrhizal Fungi with Root Organs, Soil Biology ISBN:3540240276 p.341-375, 10.1007/3-540-27331-x_18
- Datnoff L.E., Nemec S., Pernezny K., Biological Control of Fusarium Crown and Root Rot of Tomato in Florida Using Trichoderma harzianum and Glomus intraradices, 10.1006/bcon.1995.1051
- De Jaeger, FEMS Microbiol Ecol, 73, 312 (2010a)
- De Jaeger N., Decock C., Declerck S., De la Providencia I. E., Coupling autotrophic in vitro plant cultivation system to scanning electron microscope to study plant-fungal interactions, 10.5424/sjar/201008s1-1224
- Declerck S, Strullu D.G, Plenchette C, Guillemette T, Entrapment of in vitro produced spores of Glomus versiforme in alginate beads: in vitro and in vivo inoculum potentials, 10.1016/0168-1656(96)01396-x
- Doner Landis W., Bécard Guillaume, Solubilization of gellan gels by chelation of cations, 10.1007/bf00152749
- Duffy Brion, Schouten Alexander, Raaijmakers Jos M., PATHOGENSELF-DEFENSE: Mechanisms to Counteract Microbial Antagonism, 10.1146/annurev.phyto.41.052002.095606
- Fracchia S., Mujica M.T., García-Romera I., García-Garrido J.M., Martín J., Ocampo J.A., Godeas A., 10.1023/a:1004349426315
- Fracchia, Symbiosis, 36, 162 (2004)
- Fravel D. R., Encapsulation of Potential Biocontrol Agents in an Alginate-Clay Matrix, 10.1094/phyto-75-774
- Giovannetti, Symbiosis, Mechanisms and Model Systems, 295 (2002)
- Green, Appl Environ Microbiol, 65, 1428 (1999)
- Gryndler Milan, Interactions of arbuscular mycorrhizal fungi with other soil organisms, Arbuscular Mycorrhizas: Physiology and Function (2000) ISBN:9789048155156 p.239-262, 10.1007/978-94-017-0776-3_11
- Guillemin J.P., Orozco M.O., Gianinazzi-Pearson V., Gianinazzi S., Influence of phosphate fertilization on fungal alkaline phosphatase and succinate dehydrogenase activities in arbuscular mycorrhiza of soybean and pineapple, 10.1016/0167-8809(94)00555-s
- Hanlon R. D. G., Influence of Grazing by Collembola on the Activity of Senescent Fungal Colonies Grown on Media of Different Nutrient Concentration, 10.2307/3544634
- Harman Gary E., Howell Charles R., Viterbo Ada, Chet Ilan, Lorito Matteo, Trichoderma species — opportunistic, avirulent plant symbionts, 10.1038/nrmicro797
- van der Heijden Marcel G. A., Bardgett Richard D., van Straalen Nico M., The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems, 10.1111/j.1461-0248.2007.01139.x
- Hodge A., Helgason T., Fitter A.H., Nutritional ecology of arbuscular mycorrhizal fungi, 10.1016/j.funeco.2010.02.002
- Jansa Jan, Gryndler Milan, Biotic Environment of the Arbuscular Mycorrhizal Fungi in Soil, Arbuscular Mycorrhizas: Physiology and Function (2010) ISBN:9789048194889 p.209-236, 10.1007/978-90-481-9489-6_10
- Kaewchai, Fungal Divers, 38, 25 (2009)
- Lee Pau-Ju, Koske R.E., Gigaspora gigantea: parasitism of spores by fungi and actinomycetes, 10.1016/s0953-7562(09)81205-7
- Martinez Alicia, Obertello Mariana, Pardo Alejandro, Ocampo Juan A., Godeas Alicia, Interactions between Trichoderma pseudokoningii strains and the arbuscular mycorrhizal fungi Glomus mosseae and Gigaspora rosea, 10.1007/s00572-003-0240-y
- McAllister C.B., García-Romera I., Godeas A., Ocampo J.A., Interactions between Trichoderma koningii, Fusarium solani and Glomus mosseae: Effects on plant growth, arbuscular mycorrhizas and the saprophyte inoculants, 10.1016/0038-0717(94)90218-6
- McAllister C.B., García-Romera I., Godeas A., Ocampo J.A., In vitro interactions between Trichoderma koningii, Fusarium solani and Glomus mosseae, 10.1016/0038-0717(94)90219-4
- McGONIGLE T. P., MILLER M. H., EVANS D. G., FAIRCHILD G. L., SWAN J. A., A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi, 10.1111/j.1469-8137.1990.tb00476.x
- Phillips J.M., Hayman D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection, 10.1016/s0007-1536(70)80110-3
- Plenchette Christian, Strullu Désiré G., Long-term viability and infectivity of intraradical forms of Glomus intraradices vesicles encapsulated in alginate beads, 10.1017/s0953756203007482
- Pringle Anne, Taylor John W., The fitness of filamentous fungi, 10.1016/s0966-842x(02)02447-2
- Rillig Matthias C., Mummey Daniel L., Mycorrhizas and soil structure, 10.1111/j.1469-8137.2006.01750.x
- Rincón A. M., Benítez T., Codón A. C., Moreno-Mateos M. A., Biotechnological aspects of Trichoderma spp., Applied mycology ISBN:9781845935344 p.216-238, 10.1079/9781845935344.0216
- SAITO MASANORI, Enzyme activities of the internal hyphae and germinated spores of an arbuscular mycorrhizal fungus, Gigaspora margarita Becker & Hall, 10.1111/j.1469-8137.1995.tb04313.x
- Sanyal, J Biol Cont, 17, 69 (2003)
- Smith, Mycorrhizal Symbiosis (2008)
- Tisserant B., Gianinazzi-Pearson V., Gianinazzi S., Gollotte A., In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections, 10.1016/s0953-7562(09)80248-7
- Vassilev Nikolay, Vassileva Maria, Azcon Rosario, Medina Almudena, 10.1023/a:1010395813017
- Voets Liesbeth, Dupré de Boulois Hervé, Renard Laurent, Strullu Désiré-Georges, Declerck Stéphane, Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets, 10.1016/j.femsle.2005.05.025
- Weber Jean, Ducousso Marc, Tham Foong Yee, Nourissier-Mountou Sophie, Galiana Antoine, Prin Yves, Lee Sing Kong, Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture, 10.1007/s00374-005-0833-z
- Whipps John M, Prospects and limitations for mycorrhizas in biocontrol of root pathogens, 10.1139/b04-082
Bibliographic reference |
De Jaeger, Nathalie ; De La Providencia Sanchez, Ivan Enriquez ; Rouhier, H. ; Declerck, Stephan. Co-entrapment of Trichoderma harzianum and Glomus sp. within alginate beads: impact on the arbuscular mycorrhizal fungi life cycle. In: Journal of Applied Microbiology, Vol. 111, no.1, p. 125-135 (2011) |
Permanent URL |
http://hdl.handle.net/2078.1/162397 |