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Versatile Antagonistic Activities of Soil-Borne Bacillus spp. and Pseudomonas spp. against Phytophthora infestans and Other Potato Pathogens
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- 3.31 M
Caulier, Simon
[UCL]
Gillis, Annika
[UCL]
Colau, Gil
[UCL]
Licciardi, Florent
[UCL]
Liépin, Maxime
[UCL]
Desoignies, Nicolas
[UCL]
Modrie, Pauline
[UCL]
Legrève, Anne
[UCL]
Mahillon, Jacques
[UCL]
Bragard, Claude
[UCL]
Colau, Gil
[UCL]
Licciardi, Florent
[UCL]
Liépin, Maxime
[UCL]
Desoignies, Nicolas
[UCL]
Modrie, Pauline
[UCL]
Legrève, Anne
[UCL]
Mahillon, Jacques
[UCL]
Bragard, Claude
[UCL]
The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of Bacillus-like and Pseudomonas-like were isolated from several soils and substrates associated with potato agro-systems in Belgium. Screenings for antagonistic activities against the potato pathogens Alternaria solani, Fusarium solani (BCCM-MUCL 5492), Pectobacterium carotovorum (ATCC 15713), Phytophthora infestans (CRA-W10022) and Rhizoctonia solani (BCCM-MUCL 51929) were performed, allowing the selection of 52 Bacillus spp. and eight Pseudomonas spp. displaying growth inhibition of at least 50% under in vitro conditions, particularly against P. infestans. All 60 bacterial isolates were identified based on 16S rRNA gene sequencing and further characterized for the production of potential bio-active secondary metabolites. The antagonistic activities displayed by the selected strains indicated that versatile metabolites can be produced by the strains. For instance, the detection of genes involved bacilysin biosynthesis was correlated with the strong antagonism of Bacillus pumilus strains toward P. infestans, whereas the production of both bio-surfactants and siderophores might explain the high antagonistic activities against late blight. Greenhouse assays with potato plants were performed with the most effective strains (seven Bacillus spp. and four Pseudomonas spp.) in order to evaluate their in vivo antagonistic effect against P. infestans. Based on these results, four strains (Bacillus amyloliquefaciens 17A-B3, Bacillus subtilis 30B-B6, Pseudomonas brenneri 43R-P1 and Pseudomonas protegens 44R-P8) were retained for further evaluation of their protection index against P. infestans in a pilot field trial. Interestingly, B. subtilis 30B-B6 was shown to significantly decrease late blight severity throughout the crop season. Overall, this study showed that antagonistic indigenous soil bacteria can offer an alternative to the indiscriminate use of pesticide in potato agro-systems.
| Document type | Article de périodique (Journal article) – Article de recherche |
|---|---|
| Access type | Accès libre |
| Publication date | 2018 |
| Language | Anglais |
| Journal information | "Frontiers in Microbiology" - Vol. 9, p. 143 (2018) |
| Peer reviewed | yes |
| Publisher | Frontiers Media SA |
| e-issn | 1664-302X |
| Publication status | Publié |
| Affiliation | UCL - SST/ELI/ELIM - Applied Microbiology |
| Keywords | Microbiology (medical) ; Microbiology ; Bacillus spp. ; bacilysin ; bio-control ; lipopeptides ; potato diseases ; Phytophthora infestans ; Pseudomonas spp. ; siderophores |
| Links |
- Ahmadzadeh Masoud, Sharifi Tehrani Abbas, Evaluation of fluorescent pseudomonads for plant growth promotion, antifungal activity against Rhizoctonia solani on common bean, and biocontrol potential, 10.1016/j.biocontrol.2008.10.012
- Arguelles-Arias Anthony, Ongena Marc, Halimi Badre, Lara Yannick, Brans Alain, Joris Bernard, Fickers Patrick, Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens, 10.1186/1475-2859-8-63
- Ariffin, Int. J. Eng. Technol, 3, 47 (2006)
- Bach Evelise, Seger Guilherme Dubal dos Santos, Fernandes Gabriela de Carvalho, Lisboa Bruno Brito, Passaglia Luciane Maria Pereira, Evaluation of biological control and rhizosphere competence of plant growth promoting bacteria, 10.1016/j.apsoil.2015.11.002
- Bakker Peter A. H. M., Pieterse Corné M. J., van Loon L. C., Induced Systemic Resistance by FluorescentPseudomonasspp., 10.1094/phyto-97-2-0239
- Bargabus R.L, Zidack N.K, Sherwood J.E, Jacobsen B.J, Characterisation of systemic resistance in sugar beet elicited by a non-pathogenic, phyllosphere-colonizing Bacillus mycoides, biological control agent, 10.1006/pmpp.2003.0443
- Brannen P M, Kenney D S, Kodiak � --a successful biological-control product for suppression of soil-borne plant pathogens of cotton, 10.1038/sj.jim.2900439
- Bravo Alejandra, Likitvivatanavong Supaporn, Gill Sarjeet S., Soberón Mario, Bacillus thuringiensis: A story of a successful bioinsecticide, 10.1016/j.ibmb.2011.02.006
- Campbell, Introduction to Plant Disease Epidemiology (1990)
- CHMARA H., Inhibition of Glucosamine Synthase by Bacilysin and Anticapsin, 10.1099/00221287-131-2-265
- Chung Soohee, Kong Hyesuk, Buyer Jeffrey S., Lakshman Dilip K., Lydon John, Kim Sang-Dal, Roberts Daniel P., Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper, 10.1007/s00253-008-1520-4
- Clinckemaillie A., Decroës A., van Aubel G., Carrola dos Santos S., Renard M. E., Van Cutsem P., Legrève A., The novel elicitor COS-OGA enhances potato resistance to late blight, 10.1111/ppa.12641
- Cooke L. R., Schepers H. T. A. M., Hermansen A., Bain R. A., Bradshaw N. J., Ritchie F., Shaw D. S., Evenhuis A., Kessel G. J. T., Wander J. G. N., Andersson B., Hansen J. G., Hannukkala A., Nærstad R., Nielsen B. J., Epidemiology and Integrated Control of Potato Late Blight in Europe, 10.1007/s11540-011-9187-0
- Cronin, Appl. Environ. Microbiol., 63, 1357 (1997)
- Day M., Ibrahim M., Dyer D., Bulla L., Genome Sequence of Bacillus thuringiensis subsp. kurstaki Strain HD-1, 10.1128/genomea.00613-14
- de Boer Marjan, Bom Peter, Kindt Frodo, Keurentjes Joost J. B., van der Sluis Ientse, van Loon L. C., Bakker Peter A. H. M., Control of Fusarium Wilt of Radish by Combining Pseudomonas putida Strains that have Different Disease-Suppressive Mechanisms, 10.1094/phyto.2003.93.5.626
- de Bruijn Irene, de Kock Maarten J. D., Yang Meng, de Waard Pieter, van Beek Teris A., Raaijmakers Jos M., Genome-based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics inPseudomonasspecies : Cyclic lipopeptides inPseudomonas, 10.1111/j.1365-2958.2006.05525.x
- de Souza J. T., de Boer M., de Waard P., van Beek T. A., Raaijmakers J. M., Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens, 10.1128/aem.69.12.7161-7172.2003
- De Vrieze Mout, Pandey Piyush, Bucheli Thomas D., Varadarajan Adithi R., Ahrens Christian H., Weisskopf Laure, Bailly Aurélien, Volatile Organic Compounds from Native Potato-associated Pseudomonas as Potential Anti-oomycete Agents, 10.3389/fmicb.2015.01295
- Emmert Elizabeth A.B, Handelsman Jo, Biocontrol of plant disease: a (Gram-) positive perspective, 10.1111/j.1574-6968.1999.tb13405.x
- Finnan S., Morrissey J. P., O'Gara F., Boyd E. F., Genome Diversity of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients and the Hospital Environment, 10.1128/jcm.42.12.5783-5792.2004
- Gao Zhenfeng, Zhang Baojun, Liu Huiping, Han Jucai, Zhang Yongjie, Identification of endophytic Bacillus velezensis ZSY-1 strain and antifungal activity of its volatile compounds against Alternaria solani and Botrytis cinerea, 10.1016/j.biocontrol.2016.11.007
- Glare Travis, Caradus John, Gelernter Wendy, Jackson Trevor, Keyhani Nemat, Köhl Jürgen, Marrone Pamela, Morin Louise, Stewart Alison, Have biopesticides come of age?, 10.1016/j.tibtech.2012.01.003
- Grenville-Briggs L. J., Anderson V. L., Fugelstad J., Avrova A. O., Bouzenzana J., Williams A., Wawra S., Whisson S. C., Birch P. R.J., Bulone V., van West P., Cellulose Synthesis in Phytophthora infestans Is Required for Normal Appressorium Formation and Successful Infection of Potato, 10.1105/tpc.107.052043
- Haas Dieter, Défago Geneviève, Biological control of soil-borne pathogens by fluorescent pseudomonads, 10.1038/nrmicro1129
- Handelsman, Appl. Environ. Microbiol., 56, 713 (1990)
- Georgescu Mihaela, Gheorghe Irina, Curutiu Carmen, Lazar Veronica, Bleotu Coralia, Chifiriuc Mariana-Carmen, Virulence and resistance features of Pseudomonas aeruginosa strains isolated from chronic leg ulcers, 10.1186/s12879-016-1396-3
- Huang Zhengyu, Bonsall Robert F, Mavrodi Dmitri V, Weller David M, Thomashow Linda S, Transformation of Pseudomonas fluorescens with genes for biosynthesis of phenazine-1-carboxylic acid improves biocontrol of rhizoctonia root rot and in situ antibiotic production, 10.1016/j.femsec.2004.03.010
- Hussain, Afr. J. Microbiol. Res., 3, 704 (2009)
- Iavicoli Annalisa, Boutet Emmanuel, Buchala Antony, Métraux Jean-Pierre, Induced Systemic Resistance inArabidopsis thalianain Response to Root Inoculation withPseudomonas fluorescensCHA0, 10.1094/mpmi.2003.16.10.851
- Ihaka, J. Comput. Graph. Stat., 5, 299 (1996)
- Jacobsen B. J., Zidack N. K., Larson B. J., The Role of Bacillus-Based Biological Control Agents in Integrated Pest Management Systems: Plant Diseases, 10.1094/phyto.2004.94.11.1272
- Jensen G. B., Hansen B. M., Eilenberg J., Mahillon J., The hidden lifestyles of Bacillus cereus and relatives : The hidden lifestyles of B. cereus and relatives, 10.1046/j.1462-2920.2003.00461.x
- Jourdan E., Henry G., Duby F., Dommes J., Barthélemy J. P., Thonart P., Ongena M., Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide fromBacillus subtilis, 10.1094/mpmi-22-4-0456
- Kai Marco, Effmert Uta, Berg Gabriele, Piechulla Birgit, Volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen Rhizoctonia solani, 10.1007/s00203-006-0199-0
- Siddiqui Imran A, Shahid Shaukat S, Suppression of root-knot disease by Pseudomonas fluorescens CHA0 in tomato: importance of bacterial secondary metabolite, 2,4-diacetylpholoroglucinol, 10.1016/j.soilbio.2003.08.006
- Khalifa, Ann. Biol. Clin., 69, 393 (2011)
- Kloepper Joseph W., Leong John, Teintze Martin, Schroth Milton N., Pseudomonas siderophores: A mechanism explaining disease-suppressive soils, 10.1007/bf02602840
- Kloepper J.W., Reddy M.S., Rodríguez-Kabana R., Kenney D.S., Kokalis-Burelle N., Martinez-Ochoa N., APPLICATION FOR RHIZOBACTERIA IN TRANSPLANT PRODUCTION AND YIELD ENHANCEMENT, 10.17660/actahortic.2004.631.28
- Kong Hyun-Gi, Kim Jin-Cheol, Choi Gyoung-Ja, Lee Kwang-Youll, Kim Hyun-Ju, Hwang Eul-Chul, Moon Byung-Ju, Lee Seon-Woo, Production of Surfactin and Iturin by Bacillus licheniformis N1 Responsible for Plant Disease Control Activity, 10.5423/ppj.2010.26.2.170
- Lahlali R., Peng G., Gossen B. D., McGregor L., Yu F. Q., Hynes R. K., Hwang S. F., McDonald M. R., Boyetchko S. M., Evidence that the Biofungicide Serenade (Bacillus subtilis) Suppresses Clubroot on Canola via Antibiosis and Induced Host Resistance, 10.1094/phyto-06-12-0123-r
- Lanotte P., Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins, 10.1099/jmm.0.05324-0
- Leeman M., Iron Availability Affects Induction of Systemic Resistance to Fusarium Wilt of Radish byPseudomonas fluorescens, 10.1094/phyto-86-149
- Lim Jong-Hui, Synergistic Plant Growth Promotion by the Indigenous Auxins-producing PGPR Bacillus subtilis AH18 and Bacillus licheniforims K11, 10.3839/jksabc.2009.090
- Locke, Compendium of Potato Diseases, 134 (2002)
- Loper Joyce E., Current ReviewSiderophores in Microbial Interactions on Plant Surfaces, 10.1094/mpmi-4-005
- Lozano Gabriel L., Holt Jonathan, Ravel Jacques, Rasko David A., Thomas Michael G., Handelsman Jo, Draft Genome Sequence of Biocontrol AgentBacillus cereusUW85, 10.1128/genomea.00910-16
- Lund Terje, De Buyser Marie-Laure, Granum Per Einar, A new cytotoxin from Bacillus cereus that may cause necrotic enteritis, 10.1046/j.1365-2958.2000.02147.x
- Maget-Dana R., Ptak M., Peypoux F., Michel G., Pore-forming properties of iturin A, a lipopeptide antibiotic, 10.1016/0005-2736(85)90367-0
- Mavrodi D. V., Peever T. L., Mavrodi O. V., Parejko J. A., Raaijmakers J. M., Lemanceau P., Mazurier S., Heide L., Blankenfeldt W., Weller D. M., Thomashow L. S., Diversity and Evolution of the Phenazine Biosynthesis Pathway, 10.1128/aem.02009-09
- MEZIANE HAMID, VAN DER SLUIS IENTSE, VAN LOON LEENDERT C., HÖFTE MONICA, BAKKER PETER A. H. M., Determinants ofPseudomonas putidaWCS358 involved in inducing systemic resistance in plants, 10.1111/j.1364-3703.2005.00276.x
- Milewski Slawomir, Chmara Henryk, Borowski Edward, Antibiotic tetaine ? a selective inhibitor of chitin and mannoprotein biosynthesis in Candida albicans, 10.1007/bf00443651
- Naranjo M., Denayer S., Botteldoorn N., Delbrassinne L., Veys J., Waegenaere J., Sirtaine N., Driesen R. B., Sipido K. R., Mahillon J., Dierick K., Sudden Death of a Young Adult Associated with Bacillus cereus Food Poisoning, 10.1128/jcm.05129-11
- Neilands J B, Iron Absorption and Transport in Microorganisms, 10.1146/annurev.nu.01.070181.000331
- Nihorimbere Venant, Cawoy Hélène, Seyer Alexandre, Brunelle Alain, Thonart Philippe, Ongena Marc, Impact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciensS499, 10.1111/j.1574-6941.2011.01208.x
- Ongena Marc, Jacques Philippe, Bacillus lipopeptides: versatile weapons for plant disease biocontrol, 10.1016/j.tim.2007.12.009
- Ongena Marc, Jacques Philippe, Touré Yacine, Destain Jacqueline, Jabrane Abdelhamid, Thonart Philippe, Involvement of fengycin-type lipopeptides in the multifaceted biocontrol potential of Bacillus subtilis, 10.1007/s00253-005-1940-3
- Ongena Marc, Jourdan Emmanuel, Adam Akram, Paquot Michel, Brans Alain, Joris Bernard, Arpigny Jean-Louis, Thonart Philippe, Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants, 10.1111/j.1462-2920.2006.01202.x
- Raaijmakers Jos M., De Bruijn Irene, Nybroe Ole, Ongena Marc, Natural functions of lipopeptides fromBacillusandPseudomonas: more than surfactants and antibiotics, 10.1111/j.1574-6976.2010.00221.x
- Ravensberg Willem J., A Roadmap to the Successful Development and Commercialization of Microbial Pest Control Products for Control of Arthropods, ISBN:9789400704367, 10.1007/978-94-007-0437-4
- Ryu C.-M., Bacterial Volatiles Induce Systemic Resistance in Arabidopsis, 10.1104/pp.103.026583
- Ryu C.-M., Farag M. A., Hu C.-H., Reddy M. S., Wei H.-X., Pare P. W., Kloepper J. W., Bacterial volatiles promote growth in Arabidopsis, 10.1073/pnas.0730845100
- Sharma A., Shankhdhar D., SC Shankhdhar, Enhancing grain iron content of rice by the application of plant growth promoting rhizobacteria, 10.17221/683/2012-pse
- Siegmund Inka, Wagner Fritz, New method for detecting rhamnolipids excreted byPseudomonas species during growth on mineral agar, 10.1007/bf02438660
- Tamura Koichiro, Stecher Glen, Peterson Daniel, Filipski Alan, Kumar Sudhir, MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0, 10.1093/molbev/mst197
- Thomashow, Principles of Plant-Microbe Interactions: Microbes for Sustainable Agriculture, 165 (2015)
- Toure Y., Ongena M., Jacques P., Guiro A., Thonart P., Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple, 10.1111/j.1365-2672.2004.02252.x
- Tran Ha, Ficke Andrea, Asiimwe Theodore, Höfte Monica, Raaijmakers Jos M., Role of the cyclic lipopeptide massetolide A in biological control of Phytophthora infestans and in colonization of tomato plants by Pseudomonas fluorescens, 10.1111/j.1469-8137.2007.02138.x
- Verbon Eline H., Trapet Pauline L., Stringlis Ioannis A., Kruijs Sophie, Bakker Peter A.H.M., Pieterse Corné M.J., Iron and Immunity, 10.1146/annurev-phyto-080516-035537
- Vinale Francesco, Nigro Marco, Sivasithamparam Krishnapillai, Flematti Gavin, Ghisalberti Emilio L., Ruocco Michelina, Varlese Rosaria, Marra Roberta, Lanzuise Stefania, Eid Ahmed, Woo Sheridan L., Lorito Matteo, Harzianic acid: a novel siderophore fromTrichoderma harzianum, 10.1111/1574-6968.12231
- Wang L.-T., Lee F.-L., Tai C.-J., Kasai H., Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA DNA hybridization in the Bacillus subtilis group, 10.1099/ijs.0.64685-0
- Wei, R Package ‘corrplot’: Visualization of a Correlation Matrix (2016)
- Weller David M., Raaijmakers Jos M., Gardener Brian B. McSpadden, Thomashow Linda S., MICROBIALPOPULATIONSRESPONSIBLE FORSPECIFICSOILSUPPRESSIVENESS TOPLANTPATHOGENS, 10.1146/annurev.phyto.40.030402.110010
- Yang Ming-Ming, Wen Shan-Shan, Mavrodi Dmitri V., Mavrodi Olga V., von Wettstein Diter, Thomashow Linda S., Guo Jian-Hua, Weller David M., Biological Control of Wheat Root Diseases by the CLP-Producing StrainPseudomonas fluorescensHC1-07, 10.1094/phyto-05-13-0142-r
- Mounia YOUCEF-ALI, Noreddine KACEM CHAOUCHE, Laid DEHIMAT, Insaf BATAICHE, Mounira KARA ALI, Hlne CAWOY, Philippe THONART, Antifungal activity and bioactive compounds produced by Bacillus mojavensis and Bacillus subtilis, 10.5897/ajmr2013.6327
- Youssef Noha H., Duncan Kathleen E., Nagle David P., Savage Kristen N., Knapp Roy M., McInerney Michael J., Comparison of methods to detect biosurfactant production by diverse microorganisms, 10.1016/j.mimet.2003.11.001
- Yu G.Y, Sinclair J.B, Hartman G.L, Bertagnolli B.L, Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani, 10.1016/s0038-0717(02)00027-5
- Yu Xianmei, Ai Chengxiang, Xin Li, Zhou Guangfang, The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper, 10.1016/j.ejsobi.2010.11.001
| Bibliographic reference | Caulier, Simon ; Gillis, Annika ; Colau, Gil ; Licciardi, Florent ; Liépin, Maxime ; et. al. Versatile Antagonistic Activities of Soil-Borne Bacillus spp. and Pseudomonas spp. against Phytophthora infestans and Other Potato Pathogens. In: Frontiers in Microbiology, Vol. 9, p. 143 (2018) |
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| Permanent URL | http://hdl.handle.net/2078.1/195117 |