Pachikian, Barbara D.
[UCL]
Druart, Céline
[UCL]
Catry, Emilie
[UCL]
Bindels, Laure B.
[UCL]
Neyrinck, Audrey M.
[UCL]
Larondelle, Yvan
[UCL]
Cani, Patrice D.
[UCL]
Delzenne, Nathalie M.
[UCL]
(eng)
SCOPE: Conjugated linoleic acids are linoleic acid isomers found in the diet that can also be produced through bacterial metabolism of polyunsaturated fatty acids. Our objective was to evaluate the contribution of fatty acid metabolites produced from polyunsaturated fatty acids by the gut microbiota in vivo to regulation of hepatic lipid metabolism and steatosis.
METHODS AND RESULTS: In mice with depleted n-3 polyunsaturated fatty acids, we observed an accumulation of trans-11,trans-13 CLA and cis-9,cis-11 conjugated linoleic acids in the liver tissue that were associated with an increased triglyceride content and expression of lipogenic genes. We used an in vitro model to evaluate the impact of these two conjugated linoleic acids on hepatic lipid metabolism. In HepG2 cells, we observed that only trans-11,trans-13 conjugated linoleic acids recapitulated triglyceride accumulation and increased lipogenic gene expression, which is a phenomenon that may implicate the nuclear factors sterol regulatory element binding protein 1c (SREBP-1c) and carbohydrate-responsive element-binding protein (ChREBP).
CONCLUSION: The trans-11,trans-13 conjugated linoleic acids can stimulate hepatic lipogenesis, which supports the conclusion that gut microbiota and related metabolites should be considered in the treatment of non-alcoholic liver disease.
- Sattar N., Forrest E., Preiss D., Non-alcoholic fatty liver disease, 10.1136/bmj.g4596
- A Berlanga, Clin Exp Gastroenterol, 7, 221 (2014)
- Liu Wensheng, Baker Robert D., Bhatia Tavleen, Zhu Lixin, Baker Susan S., Pathogenesis of nonalcoholic steatohepatitis, 10.1007/s00018-016-2161-x
- Delarue Jacques, Lallès Jean-Paul, Nonalcoholic fatty liver disease: Roles of the gut and the liver and metabolic modulation by some dietary factors and especially long-chain n-3 PUFA, 10.1002/mnfr.201500346
- Backhed F., Ding H., Wang T., Hooper L. V., Koh G. Y., Nagy A., Semenkovich C. F., Gordon J. I., The gut microbiota as an environmental factor that regulates fat storage, 10.1073/pnas.0407076101
- Ducheix Simon, Montagner Alexandra, Theodorou Vassilia, Ferrier Laurent, Guillou Hervé, The liver X receptor: A master regulator of the gut–liver axis and a target for non alcoholic fatty liver disease, 10.1016/j.bcp.2013.03.016
- Tan Nguan Soon, Vázquez-Carrera Manuel, Montagner Alexandra, Sng Ming Keat, Guillou Hervé, Wahli Walter, Transcriptional control of physiological and pathological processes by the nuclear receptor PPARβ/δ, 10.1016/j.plipres.2016.09.001
- Delzenne Nathalie M., Cani Patrice D., Everard Amandine, Neyrinck Audrey M., Bindels Laure B., Gut microorganisms as promising targets for the management of type 2 diabetes, 10.1007/s00125-015-3712-7
- Raimondi Stefano, Amaretti Alberto, Leonardi Alan, Quartieri Andrea, Gozzoli Caterina, Rossi Maddalena, Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition, 10.1155/2016/8654317
- Druart Céline, Bindels Laure B., Schmaltz Robert, Neyrinck Audrey M., Cani Patrice D., Walter Jens, Ramer-Tait Amanda E., Delzenne Nathalie M., Ability of the gut microbiota to produce PUFA-derived bacterial metabolites: Proof of concept in germ-free versus conventionalized mice, 10.1002/mnfr.201500014
- Druart Céline, Neyrinck Audrey M., Vlaeminck Bruno, Fievez Veerle, Cani Patrice D., Delzenne Nathalie M., Role of the Lower and Upper Intestine in the Production and Absorption of Gut Microbiota-Derived PUFA Metabolites, 10.1371/journal.pone.0087560
- Kim Jun Ho, Kim Yoo, Kim Young Jun, Park Yeonhwa, Conjugated Linoleic Acid: Potential Health Benefits as a Functional Food Ingredient, 10.1146/annurev-food-041715-033028
- Lee Yong-Jae, Jenkins Thomas C., Biohydrogenation of Linolenic Acid to Stearic Acid by the Rumen Microbial Population Yields Multiple Intermediate Conjugated Diene Isomers, 10.3945/jn.111.138396
- Honkanen Anne M., Leskinen Heidi, Toivonen Vesa, McKain Nest, Wallace R. John, Shingfield Kevin J., Metabolism of α-linolenic acid during incubations with strained bovine rumen contents: products and mechanisms, 10.1017/s0007114516001446
- Russell D.A., Ross R.P., Fitzgerald G.F., Stanton C., Metabolic activities and probiotic potential of bifidobacteria, 10.1016/j.ijfoodmicro.2011.06.003
- McIntosh F. M., Shingfield K. J., Devillard E., Russell W. R., Wallace R. J., Mechanism of conjugated linoleic acid and vaccenic acid formation in human faecal suspensions and pure cultures of intestinal bacteria, 10.1099/mic.0.022921-0
- O'Shea Eileen F., Cotter Paul D., Stanton Catherine, Ross R. Paul, Hill Colin, Production of bioactive substances by intestinal bacteria as a basis for explaining probiotic mechanisms: Bacteriocins and conjugated linoleic acid, 10.1016/j.ijfoodmicro.2011.05.025
- Jenkins T. C., Wallace R. J., Moate P. J., Mosley E. E., BOARD-INVITED REVIEW: Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem1, 10.2527/jas.2007-0588
- Pini Maria, Touch Sothea, Poirier Hélène, Dalmas Elise, Niot Isabelle, Rouault Christine, Druart Céline, Delzenne Nathalie, Clément Karine, André Sébastien, Guerre-Millo Michèle, Adipose tissue adaptive response to trans-10,cis-12–conjugated linoleic acid engages alternatively activated M2 macrophages, 10.1096/fj.15-276675
- Shen Wan, Chuang Chia-Chi, Martinez Kristina, Reid Tanya, Brown J. Mark, Xi Lin, Hixson Lindsay, Hopkins Robin, Starnes Joseph, McIntosh Michael, Conjugated linoleic acid reduces adiposity and increases markers of browning and inflammation in white adipose tissue of mice, 10.1194/jlr.m030924
- Go Gwang-woong, t10,c12 Conjugated Linoleic Acid Upregulates Hepatic De Novo Lipogenesis and Triglyceride Synthesis via mTOR Pathway Activation, 10.4014/jmb.1308.08008
- Mitchell Patricia L., Karakach Tobias K., Currie Deborah L., McLeod Roger S., t-10, c-12 CLA Dietary Supplementation Inhibits Atherosclerotic Lesion Development Despite Adverse Cardiovascular and Hepatic Metabolic Marker Profiles, 10.1371/journal.pone.0052634
- Della Casa L., Rossi E., Romanelli C., Gibellini L., Iannone A., Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice, 10.1186/s12263-016-0542-2
- Ferramosca Alessandra, Modulation of hepatic steatosis by dietary fatty acids, 10.3748/wjg.v20.i7.1746
- SY Moya-Camarena, J Lipid Res, 40, 1426 (1999)
- Marques Tatiana M., Wall Rebecca, O'Sullivan Orla, Fitzgerald Gerald F., Shanahan Fergus, Quigley Eamonn M., Cotter Paul D., Cryan John F., Dinan Timothy G., Ross R. Paul, Stanton Catherine, Dietary trans-10, cis-12-conjugated linoleic acid alters fatty acid metabolism and microbiota composition in mice, 10.1017/s0007114514004206
- Pachikian Barbara D., Essaghir Ahmed, Demoulin Jean-Baptiste, Catry Emilie, Neyrinck Audrey M., Dewulf Evelyne M., Sohet Florence M., Portois Laurence, Clerbaux Laure-Alix, Carpentier Yvon A., Possemiers Sam, Bommer Guido T., Cani Patrice D., Delzenne Nathalie M., Prebiotic approach alleviates hepatic steatosis: Implication of fatty acid oxidative and cholesterol synthesis pathways, 10.1002/mnfr.201200364
- Pachikian Barbara D., Essaghir Ahmed, Demoulin Jean-Baptiste, Neyrinck Audrey M., Catry Emilie, De Backer Fabienne C., Dejeans Nicolas, Dewulf Evelyne M., Sohet Florence M., Portois Laurence, Deldicque Louise, Molendi-Coste Olivier, Leclercq Isabelle A., Francaux Marc, Carpentier Yvon A., Foufelle Fabienne, Muccioli Giulio G., Cani Patrice D., Delzenne Nathalie M., Hepatic n-3 Polyunsaturated Fatty Acid Depletion Promotes Steatosis and Insulin Resistance in Mice: Genomic Analysis of Cellular Targets, 10.1371/journal.pone.0023365
- Lee Yunkyoung, Vanden Heuvel John P., Inhibition of macrophage adhesion activity by 9trans,11trans-conjugated linoleic acid, 10.1016/j.jnutbio.2009.02.005
- J Folch, J Biol Chem, 226, 497 (1957)
- Bligh E. G., Dyer W. J., A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION, 10.1139/o59-099
- Pachikian BD, Neyrinck AM, Cani PD, Portois L, Deldicque L, De Backer FC, Bindels LB, Sohet FM, Malaisse WJ, Francaux M, Carpentier YA, Delzenne NM, Hepatic steatosis in n-3 fatty acid depleted mice: focus on metabolic alterations related to tissue fatty acid composition, 10.1186/1472-6793-8-21
- Marchesi Julian R, Adams David H, Fava Francesca, Hermes Gerben D A, Hirschfield Gideon M, Hold Georgina, Quraishi Mohammed Nabil, Kinross James, Smidt Hauke, Tuohy Kieran M, Thomas Linda V, Zoetendal Erwin G, Hart Ailsa, The gut microbiota and host health: a new clinical frontier, 10.1136/gutjnl-2015-309990
- H Tilg, Gut (2016)
- Clarke Gerard, Stilling Roman M., Kennedy Paul J., Stanton Catherine, Cryan John F., Dinan Timothy G., Minireview: Gut Microbiota: The Neglected Endocrine Organ, 10.1210/me.2014-1108
- W Cui, Am J Transl Res, 2, 95 (2010)
- Nagao Koji, Inoue Nao, Wang Yu-Ming, Shirouchi Bungo, Yanagita Teruyoshi, Dietary Conjugated Linoleic Acid Alleviates Nonalcoholic Fatty Liver Disease in Zucker (fa/fa) Rats, 10.1093/jn/135.1.9
- Choi Joo Sun, Koh In-Uk, Jung Myeong Ho, Song Jihyun, Effects of three different conjugated linoleic acid preparations on insulin signalling, fat oxidation and mitochondrial function in rats fed a high-fat diet, 10.1017/s000711450770497x
- Clément Lionel, Poirier Hélène, Niot Isabelle, Bocher Virginie, Guerre-Millo Michèle, Krief Stéphane, Staels Bart, Besnard Philippe, Dietarytrans-10,cis-12conjugated linoleic acid induces hyperinsulinemia and fatty liver in the mouse, 10.1194/jlr.m20008-jlr200
- Kelley Darshan S., Vemuri Madhuri, Adkins Yuriko, Gill Sher Himmat S., Fedor Dawn, Mackey Bruce E., Flaxseed oil prevents trans-10, cis-12-conjugated linoleic acid-induced insulin resistance in mice, 10.1017/s0007114508027451
- P Ferre, Horm Res, 68, 72 (2007)
- Abdul-Wahed Aya, Guilmeau Sandra, Postic Catherine, Sweet Sixteenth for ChREBP: Established Roles and Future Goals, 10.1016/j.cmet.2017.07.004
- Ducheix Simon, Montagner Alexandra, Polizzi Arnaud, Lasserre Frédéric, Marmugi Alice, Bertrand-Michel Justine, Podechard Normand, Al Saati Talal, Chétiveaux Maud, Baron Silvère, Boué Jérôme, Dietrich Gilles, Mselli-Lakhal Laila, Costet Philippe, Lobaccaro Jean-Marc A., Pineau Thierry, Theodorou Vassilia, Postic Catherine, Martin Pascal G.P., Guillou Hervé, Essential fatty acids deficiency promotes lipogenic gene expression and hepatic steatosis through the liver X receptor, 10.1016/j.jhep.2013.01.006
- Watanabe Mitsuhiro, Houten Sander M., Wang Li, Moschetta Antonio, Mangelsdorf David J., Heyman Richard A., Moore David D., Auwerx Johan, Bile acids lower triglyceride levels via a pathway involving FXR, SHP, and SREBP-1c, 10.1172/jci21025
| Bibliographic reference |
Pachikian, Barbara D. ; Druart, Céline ; Catry, Emilie ; Bindels, Laure B. ; Neyrinck, Audrey M. ; et. al. Implication of trans-11,trans-13 conjugated linoleic acid in the development of hepatic steatosis.. In: PLoS One, Vol. 13, no. 2, p. e0192447 [1-14] (2018) |
| Permanent URL |
http://hdl.handle.net/2078.1/206690 |
