Puccetti, Paolo
Bianchi, Roberta
Fioretti, Maria C.
Ayroldi, Emira
Uyttenhove, Catherine
Van Pel, Aline
[UCL]
Boon-Falleur, Thierry
[UCL]
Grohmann, Ursula
We have observed delayed-type hypersensitivity (DTH) reactions in immunized mice challenged subcutaneously with class I-binding peptides related to rejection antigens recognized by cytotoxic T lymphocytes on mutagenized (tum-) variants of mastocytoma P815. As observed by skin test in virally infected mice challenged with viral peptides, the intrafootpad injection of tum- peptides resulted in a dose-dependent DTH that peaked at approximately 24 h. The response was mediated by CD8+ cells and could be induced by previous vaccination of mice with live tumor cells, intrasplenic deposition of the eliciting peptide, or adoptive transfer with peptide-pulsed syngeneic dendritic cells. These sensitization procedures resulted in an immunologically specific footpad reaction detectable for up to 2-6 months after priming. The evaluation by DTH in cancer patients of long-lived CD8+ anti-tumor T cell responses following local challenge with tumor-specific peptides may be of great interest in human immunotherapy trials involving immunization against identified tumor antigens.
Bibliographic reference |
Puccetti, Paolo ; Bianchi, Roberta ; Fioretti, Maria C. ; Ayroldi, Emira ; Uyttenhove, Catherine ; et. al. Use of a skin test assay to determine tumor-specific CD8+ T cell reactivity. In: European Journal of Immunology, Vol. 24, no.6, p. 1446-1452 (1994) |
Permanent URL |
http://hdl.handle.net/2078.1/244518 |