Exploration of untargeted metabolomic extraction methods for in vitro malaria samples by 1HNMR analysis

Mamede, Lúcia Schoumacher, Matthieu Cirillo, Arianna Fall, Fanta [UCL] Bugli, Céline [UCL] Ledoux, Allison De Tullio, Pascal Quetin-Leclercq, Joëlle [UCL] Govaerts, Bernadette [UCL] Frédérich, Michel et al.[show all]

Metabolomics is a reliable omics tool to study the metabolome, the assortment of metabolites that provide energy, signaling or building blocks essential for biological systems survival. • Malaria is a deadly disease, especially severe when caused by Plasmodium falciparum, that still affects over 200 million people yearly.1 • The metabolome closely reflects the state of the biological system, and if applied to the P. falciparum, it can be used to characterize antimalarial mechanisms of action or study resistance. • The parasite’s intracellular nature, in red blood cells in suspension, introduces significant hurdles to metabolomics extraction methods.