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Cell Surface of Lactococcus lactis Is Covered by a Protective Polysaccharide Pellicle

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Chapot-Chartier, Marie-Pierre [INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France] Vinogradov, Evgeny [Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario KIA OR6, Canada] Sadovskaya, Irina [Universite Lille Nord de France, F-59000 Lille, France] André, Guillaume [UCL] Mistou, Michel-Yves [Institut Pasteur, Unite´ de Biologie des Bacte´ries Pathoge`nes a` Gram-Positif, CNRS, URA 2172, 75724 Paris, France] Hols, Pascal [UCL] Dufrêne, Yves [UCL] Kulakauskas, Saulius [INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France]

In Gram-positive bacteria, the functional role of surface polysaccharides (PS) that are not of capsular nature remains poorly understood. Here, we report the presence of a novel cell wall PS pellicle on the surface of Lactococcus lactis. Spontaneous PS-negative mutants were selected using semi-liquid growth conditions, and all mutations were mapped in a single chromosomal locus coding for PS biosynthesis. PS molecules were shown to be composed of hexasaccharide phosphate repeating units that are distinct from other bacterial PS. Using complementary atomic force and transmission electron microscopy techniques, we showed that the PS layer forms an outer pellicle surrounding the cell. Notably, we found that this cell wall layer confers a protective barrier against host phagocytosis by murine macrophages. Altogether, our results suggest that the PS pellicle could represent a new cell envelope structural component of Gram-positive bacteria.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2010
Language Anglais
Journal information "Journal of Biological Chemistry" - Vol. 285, no. 14, p. 10464-10471 (2010)
Peer reviewed yes
Publisher Amer Soc Biochemistry Molecular Biology Inc (Bethesda)
issn 0021-9258
e-issn 1083-351X
Publication status Publié
Affiliations UCL - SST/IMCN/BSMA - Bio and soft matter
UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
UCL - SST/IMCN - Institute of Condensed Matter and Nanosciences
Links
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Bibliographic reference Chapot-Chartier, Marie-Pierre ; Vinogradov, Evgeny ; Sadovskaya, Irina ; André, Guillaume ; Mistou, Michel-Yves ; et. al. Cell Surface of Lactococcus lactis Is Covered by a Protective Polysaccharide Pellicle. In: Journal of Biological Chemistry, Vol. 285, no. 14, p. 10464-10471 (2010)
Permanent URL http://hdl.handle.net/2078.1/33993