|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography
| Title: | Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography |
| Authors: | Takegawa, Yasuhiro Browse this author →KAKEN DB | | Deguchi, Kisaburo2 Browse this author | | Keira, Takuro Browse this author | | Ito, Hiroki Browse this author | | Nakagawa, Hiroaki Browse this author | | Nishimura, Shin-Ichiro Browse this author →KAKEN DB |
| Authors(alt): | 出口, 喜三郎2 |
| Keywords: | zwitterion-interaction column | | hydrophilic-interaction chromatography | | oligosaccharides | | glycopeptides | | mass spectrometry |
| Issue Date: | 28-Apr-2006 |
| Publisher: | Elsevier |
| Journal Title: | Journal of Chromatography A |
| Volume: | 1113 |
| Issue: | 1-2 |
| Start Page: | 177 |
| End Page: | 181 |
| Publisher DOI: | 10.1016/j.chroma.2006.02.010 |
| PMID: | 16503336 |
| Abstract: | Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction chromatography column with sulfobetaine groups (called ZIC-HILIC column) was first applied to the separation of 2-aminopyridine derivatized (PA) N-glycans and tryptic peptides of human serum immunoglobulin G (IgG). It is shown that the ZIC-HILIC column has high capability for structural recognition of isomeric N-glycans as well as high selectivity for glycopeptides. The former feature (i.e., structural recognition) was proven by sufficient separation of neutral PA N-glycan isomers, which are usually difficult to separate on NP and RP columns. In addition, it is noteworthy that IgG glycopeptides consisting of isomeric N-glycans and the same peptide sequences can be sufficiently separated on a ZIC-HILIC column. The latter feature (i.e., selectivity) was also demonstrated by easily separating two peptide groups with/without N-glycans. Thus, we note that the ZIC-HILIC column is highly promising for a simple analysis of N-glycans and N-glycopeptide samples. (c) 2006 Elsevier B.V. All rights reserved. |
| Relation: | http://www.sciencedirect.com/science/journal/00219673 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/10521 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 出口 喜三郎
|
