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Characterization and antibacterial assessments of Ag nanoclusters/rose bengal nanocomposite for antimicrobial photodynamic therapy
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| Title: | Characterization and antibacterial assessments of Ag nanoclusters/rose bengal nanocomposite for antimicrobial photodynamic therapy |
| Other Titles: | 抗菌的光線力学療法に向けた銀ナノクラスター/ローズベンガル複合体の特性並びに抗菌性評価 |
| Authors: | 蔀, 佳奈子 Browse this author |
| Keywords: | Aggregatibacter actinomycetemcomitans | | Photosensitizer | | Porphyromonas gingivalis | | Singlet oxygen | | Streptococcus mutans |
| Issue Date: | 25-Mar-2019 |
| Publisher: | Hokkaido University |
| Abstract: | Ag nanoclusters (AgNCs) / rose bengal (RB) nanocomposite (AgNCs/RB) as a novel photosensitizer was created for antibacterial photodynamic therapy (a-PDT). We proposed double antibacterial effects of photoexcited AgNCs/RB; (1) singlet oxygen (1O2) generated by irradiated RB and (2) Ag+ ion released by AgNCs oxidized by 1O2. In present study, the antimicrobial and the cytotoxic effects of AgNCs/RB were evaluated.1O2 generation, zeta potential and Ag+ release of AgNCs/RB was characterized. Subsequently, the antimicrobial effect of AgNCs/RB irradiated by white light-emitting diode (LED) was evaluated using oral bacterial cells; Streptococcus mutans, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. In addition, cytotoxicity of AgNCs/RB on NIH3T3 mammalian cells was investigated. The binding of AgNCs and RB, 1O2 generation and Ag+ ion release of photoexcited AgNCs/RB were estimated. AgNCs/RB irradiated by white LED (60 sec) showed significantly inhibited the growth of oral bacteria when compared to AgNCs or RB. In addition, photoexcited AgNCs/RB did not negatively affect the adhesion, spreading and proliferation of mammalian cells, even at concentrations that showed antibacterial activity. In conclusion, AgNCs/RB irradiated by white LED showed great antimicrobial effects via 1O2 generation and Ag+ ion releasing and low cytotoxic properties to be provided for dental a-PDT. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第13482号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 特任教授 柴田 健一郎, 教授 田村 正人, 准教授 菅谷 勉 |
| Degree Affiliation: | 歯学研究科(口腔医学専攻) |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/80729 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （歯学）
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
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