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Creatine binding in white muscle of the rainbow trout Mossey, Mark Kenneth Philip.
Abstract
The subcellular distribution of creatine (Cr) in white muscle of the rainbow trout (Oncorhynchus mykiss) was examined. Rainbow trout were cannulated via the dorsal injected with [amidino-14C] Cr. Following a 2 hour incubation period, fish were either sampled in the resting state (resting group) or exercised according to one of two different exercise protocols prior to sampling (buffering and depleting groups). The specific activity of phosphocreatine (SA[sub]PCr) was over three times the SA[sub]Cr within resting white muscle, and the SA[sub]PCr/SA[sub]Cr, ratio did not decrease towards 1 in muscle sampled from fish following either exercise protocol. This indicates; i) that a significant part of the pool of total Cr (the 'bound' pool) does not have access to creatine kinase (CK) in resting white muscle and ii) that this fraction of the Cr pool does not gain access to CK and become metabolically active during bouts of intense exercise. The SA[sub]PCr/SA[sub]Cr ratio increased significantly with decreasing Cr charge and thus the pool of Cr which was interacting with CK was not mixing homogeneously in all parts of the cell. This suggests limited subcellular mobility of PCr and Cr. The finding that a significant part of the pool of total Cr is not active in energy metabolism affects calculations of free adenosine diphosphate (ADP) through the CK equilibrium. Calculations of free ADP which take the limited access of Cr to CK into consideration imply a significant role for ADP as a regulator of metabolism.
Item Metadata
| Title |
Creatine binding in white muscle of the rainbow trout
|
| Creator | |
| Publisher |
University of British Columbia
|
| Date Issued |
1995
|
| Description |
The subcellular distribution of creatine (Cr) in white muscle of the rainbow trout
(Oncorhynchus mykiss) was examined. Rainbow trout were cannulated via the dorsal injected with [amidino-14C] Cr. Following a 2 hour incubation period, fish were either sampled in
the resting state (resting group) or exercised according to one of two different exercise protocols
prior to sampling (buffering and depleting groups). The specific activity of phosphocreatine
(SA[sub]PCr) was over three times the SA[sub]Cr within resting white muscle, and the SA[sub]PCr/SA[sub]Cr, ratio did
not decrease towards 1 in muscle sampled from fish following either exercise protocol. This
indicates; i) that a significant part of the pool of total Cr (the 'bound' pool) does not have access
to creatine kinase (CK) in resting white muscle and ii) that this fraction of the Cr pool does not
gain access to CK and become metabolically active during bouts of intense exercise. The
SA[sub]PCr/SA[sub]Cr ratio increased significantly with decreasing Cr charge and thus the pool of Cr which
was interacting with CK was not mixing homogeneously in all parts of the cell. This suggests
limited subcellular mobility of PCr and Cr.
The finding that a significant part of the pool of total Cr is not active in energy metabolism
affects calculations of free adenosine diphosphate (ADP) through the CK equilibrium.
Calculations of free ADP which take the limited access of Cr to CK into consideration imply a
significant role for ADP as a regulator of metabolism.
|
| Extent |
3085553 bytes
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| Genre | |
| Type | |
| File Format |
application/pdf
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| Language |
eng
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| Date Available |
2009-01-13
|
| Provider |
Vancouver : University of British Columbia Library
|
| Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
|
| DOI |
10.14288/1.0086812
|
| URI | |
| Degree | |
| Program | |
| Affiliation | |
| Degree Grantor |
University of British Columbia
|
| Graduation Date |
1995-05
|
| Campus | |
| Scholarly Level |
Graduate
|
| Aggregated Source Repository |
DSpace
|
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For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.