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journal.pone.0070317.pdf | 3.43 MB | Adobe PDF | 見る/開く |
タイトル: | Reconstruction of Active Regular Motion in Amoeba Extract: Dynamic Cooperation between Sol and Gel States. |
著者: | Nishigami, Yukinori Ichikawa, Masatoshi https://orcid.org/0000-0002-6345-2106 (unconfirmed) Kazama, Toshiya Kobayashi, Ryo Shimmen, Teruo Yoshikawa, Kenichi Sonobe, Seiji |
著者名の別形: | 市川, 正敏 |
発行日: | 5-Aug-2013 |
出版者: | Public Library of Science |
誌名: | PloS one |
巻: | 8 |
号: | 8 |
論文番号: | e70317 |
抄録: | Amoeboid locomotion is one of the typical modes of biological cell migration. Cytoplasmic sol-gel conversion of an actomyosin system is thought to play an important role in locomotion. However, the mechanisms underlying sol-gel conversion, including trigger, signal, and regulating factors, remain unclear. We developed a novel model system in which an actomyosin fraction moves like an amoeba in a cytoplasmic extract. Rheological study of this model system revealed that the actomyosin fraction exhibits shear banding: the sol-gel state of actomyosin can be regulated by shear rate or mechanical force. Furthermore, study of the living cell indicated that the shear-banding property also causes sol-gel conversion with the same order of magnitude as that of shear rate. Our results suggest that the inherent sol-gel transition property plays an essential role in the self-regulation of autonomous translational motion in amoeba. |
著作権等: | © 2013 Nishigami et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/178066 |
DOI(出版社版): | 10.1371/journal.pone.0070317 |
PubMed ID: | 23940560 |
出現コレクション: | 学術雑誌掲載論文等 |
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