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タイトル: | SNAIL regulates gastric carcinogenesis through CCN3 and NEFL |
その他のタイトル: | EMT関連因子SNAILは、2つの遺伝子CCN3 およびNEFLを通 じて胃発癌を制御する |
著者: | Chen, Ru Masuo, Kenji Yogo, Akitada Yokoyama, Shoko Sugiyama, Aiko Seno, Hiroshi Yoshizawa, Akihiko Takaishi, Shigeo https://orcid.org/0000-0003-2236-6081 (unconfirmed) |
著者名の別形: | 陳, 茹 増尾, 謙志 余語, 覚匡 杉山, 愛子 妹尾, 浩 吉澤, 明彦 高石, 繁生 |
キーワード: | gastric cancer cell lines snails carcinogenesis tumor cells malignant |
発行日: | Feb-2021 |
出版者: | Oxford University Press (OUP) |
誌名: | Carcinogenesis |
巻: | 49 |
号: | 2 |
開始ページ: | 190 |
終了ページ: | 201 |
抄録: | Among cancer cells, there are specific cell populations of whose activities are comparable to those of stem cells in normal tissues, and for whom the levels of cell dedifferentiation are reported to correlate with poor prognosis. Information concerning the mechanisms that modulate the stemness like traits of cancer cells is limited. Therefore, we examined five gastric cancer cell lines and isolated gastric oncospheres from three gastric cancer cell lines. The gastric cancer cells that expanded in the spheres expressed relatively elevated proportion of CD44, which is a marker of gastric cancer stem cells (CSCs), and displayed many properties of CSCs, for example: chemoresistance, tumorigenicity and epithelial–mesenchymal transition (EMT) acquisition. SNAIL, which is a key factor in EMT, was highly expressed in the gastric spheres. Microarray analysis in gastric cancer cell line HGC27 showed that CCN3 and NEFL displayed the greatest differential expression by knocking down of SNAIL; the former was upregulated and the latter downregulated, respectively. Downregulation of CCN3 and upregulation of NEFL gene expression impaired the SNAIL-dependent EMT activity: high tumorigenicity, and chemoresistance in gastric cancer cells. Thus, approach that disrupts SNAIL/CCN3/NEFL axis may be credible in inhibiting gastric cancer development. |
著作権等: | This is a pre-copyedited, author-produced version of an article accepted for publication in Carcinogenesis following peer review. The version of record Ru Chen, Kenji Masuo, Akitada Yogo, Shoko Yokoyama, Aiko Sugiyama, Hiroshi Seno, Akihiko Yoshizawa, Shigeo Takaishi, SNAIL regulates gastric carcinogenesis through CCN3 and NEFL, Carcinogenesis, Volume 42, Issue 2, February 2021, Pages 190–201 is available online at: https://doi.org/10.1093/carcin/bgaa133. The full-text file will be made open to the public on 12 December 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/261804 |
DOI(出版社版): | 10.1093/carcin/bgaa133 |
PubMed ID: | 33313663 |
出現コレクション: | 学術雑誌掲載論文等 |
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