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j.isci.2022.103987.pdf | 3.21 MB | Adobe PDF | 見る/開く |
タイトル: | One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors |
著者: | Otsuka, Yuki Imamura, Keiko Oishi, Akio Kondo, Takayuki Suga, Mika Yada, Yuichiro Shibukawa, Ran Okanishi, Yasue Sagara, Yukako Tsukita, Kayoko Tsujikawa, Akitaka Inoue, Haruhisa https://orcid.org/0000-0003-4736-9537 (unconfirmed) |
著者名の別形: | 大塚, 悠生 今村, 恵子 大石, 明生 近藤, 孝之 菅, 三佳 矢田, 祐一郎 澁川, 蘭 岡西, 泰永 佐柄, 友佳子 月田, 香代子 辻川, 明孝 井上, 治久 |
キーワード: | Molecular biology Cell biology Stem cells research |
発行日: | Apr-2022 |
出版者: | Elsevier BV |
誌名: | iScience |
巻: | 25 |
号: | 4 |
論文番号: | 103987 |
抄録: | Retinal dystrophies (RDs) lead to irreversible vision impairment with no radical treatment. Although photoreceptor cells (PRCs) differentiated from human induced pluripotent stem cells (iPSCs) are essential for the study of RDs as a scalable source, current differentiation methods for PRCs require multiple steps. To address these issues, we developed a method to generate PRCs from human iPSCs by introducing the transcription factors, CRX and NEUROD1. This approach enabled us to generate induced photoreceptor-like cells (iPRCs) expressing PRC markers. Single-cell RNA sequencing revealed the transcriptome of iPRCs in which the genes associated with phototransduction were expressed. Generated iPRCs exhibited their functional properties in calcium imaging. Furthermore, light-induced damage on iPRCs was inhibited by an antioxidant compound. This simple approach would facilitate the availability of materials for PRC-related research and provide a useful application for disease modeling and drug discovery. |
記述: | ヒトiPS細胞からの視細胞直接誘導法 --視細胞を迅速かつ簡便に分化誘導することが可能に--. 京都大学プレスリリース. 2022-03-29. |
著作権等: | © 2022 The Authors. This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
URI: | http://hdl.handle.net/2433/269077 |
DOI(出版社版): | 10.1016/j.isci.2022.103987 |
PubMed ID: | 35330684 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/220329-140000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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