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タイトル: | Population-level prokaryotic community structures associated with ferromanganese nodules in the Clarion-Clipperton Zone (Pacific Ocean) revealed by 16S rRNA gene amplicon sequencing |
著者: | Tominaga, Kento Takebe, Hiroaki Murakami, Chisato Tsune, Akira Okamura, Takahiko Ikegami, Takuji Onishi, Yosuke Kamikawa, Ryoma Yoshida, Takashi |
著者名の別形: | 富永, 賢人 武部, 紘明 神川, 龍馬 吉田, 天士 |
発行日: | Feb-2024 |
出版者: | Wiley Applied Microbiology International |
誌名: | Environmental Microbiology Reports |
巻: | 16 |
号: | 1 |
論文番号: | e13224 |
抄録: | Although deep-sea ferromanganese nodules are a potential resource for exploitation, their formation mechanisms remain unclear. Several nodule-associated prokaryotic species have been identified by amplicon sequencing of 16S rRNA genes and are assumed to contribute to nodule formation. However, the recent development of amplicon sequence variant (ASV)-level monitoring revealed that closely related prokaryotic populations within an operational taxonomic unit often exhibit distinct ecological properties. Thus, conventional species-level monitoring might have overlooked nodule-specific populations when distinct populations of the same species were present in surrounding environments. Herein, we examined the prokaryotic community diversity of nodules and surrounding environments at the Clarion-Clipperton Zone in Japanese licensed areas by 16S rRNA gene amplicon sequencing with ASV-level resolution for three cruises from 2017 to 2019. Prokaryotic community composition and diversity were distinct by habitat type: nodule, nodule-surface mud, sediment, bottom water and water column. Most ASVs (~80%) were habitat-specific. We identified 178 nodule-associated ASVs and 41 ASVs associated with nodule-surface mud via linear discriminant effect size analysis. Moreover, several ASVs, such as members of SAR324 and Woeseia, were highly specific to nodules. These nodule-specific ASVs are promising targets for future investigation of the nodule formation process. |
著作権等: | © 2023 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/287086 |
DOI(出版社版): | 10.1111/1758-2229.13224 |
PubMed ID: | 38146681 |
出現コレクション: | 学術雑誌掲載論文等 |
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