Ecotoxicology is becoming more prominent in the curricula of wildlife, biology, and environmental science fields universally. Chemicals in natural systems have posed a set of problems, thus ecotoxicology provides a broad conceptual framework for evaluating toxins in the environment. For these environmental protection studies, a number of standardized tests are available, such as the fish test, algae test, and seedling test. Under EPA guidelines I performed the seedling test.