Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/196820
Title: | Antimetastatic properties of Prodigiosin and the BH3-Mimetic Obatoclax (GX15-070) in melanoma |
Author: | Espona Fiedler, Margarita Manuel-Manresa, Pilar Benítez-García, Cristina Fontova, Pere Quesada, Roberto Soto Cerrato, Vanessa Pérez Tomás, Ricardo E. |
Keywords: | Metàstasi Melanoma Assaigs clínics de medicaments Quimioteràpia del càncer Metastasis Melanoma Drug testing Cancer chemotherapy |
Issue Date: | 28-Dec-2022 |
Publisher: | MDPI |
Abstract: | Metastasis is the primary cause of death in cancer patients. Many current chemotherapeutic agents only show cytotoxic, but not antimetastatic properties. This leads to a reduction in tumor size, but allows cancer cells to disseminate, which ultimately causes patient death. Therefore, novel anticancer compounds with both effects need to be developed. In this work, we analyze the antimetastatic properties of prodigiosin and obatoclax (GX15-070), anticancer drugs of the Prodiginines (PGs) family. We studied PGs' effects on cellular adhesion and morphology in the human primary and metastatic melanoma cell lines, SK-MEL-28 and SK-MEL-5, and in the murine melanoma cell line, B16F10A. Cell adhesion sharply decreased in the treated cells, and this was accompanied by a reduction in filopodia protrusions and a significant decrease in the number of focal-adhesion structures. Moreover, cell migration was assessed through the wound-healing assay and cell motility was severely inhibited after 24 h of treatment. To elucidate the molecular mechanisms involved, changes in metastasis-related genes were analyzed through a gene-expression array. Key genes related to cellular invasion, migration and chemoresistance were significantly down-regulated. Finally, an in vivo model of melanoma-induced lung metastasis was established and significant differences in lung tumors were observed in the obatoclax-treated mice. Altogether, these results describe, in depth, PGs' cellular antimetastatic effects and identify in vivo antimetastatic properties of Obatoclax. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/pharmaceutics15010097 |
It is part of: | Pharmaceutics, 2022, vol. 15, num. 1 |
URI: | http://hdl.handle.net/2445/196820 |
Related resource: | https://doi.org/10.3390/pharmaceutics15010097 |
ISSN: | 1999-4923 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Patologia i Terapèutica Experimental) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
728762.pdf | 3.59 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License