Publications
Detailed Information
Altered Properties of Feline Adipose Tissue-Derived Mesenchymal Stem Cells During Continuous In Vitro Cultivation : 고양이 지방 유래 중간엽 줄기세포의 지속적인 세포 외 배양에 따른 특성 변화에 대한 연구
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Advisor
- 윤화영
- Major
- 수의과대학 수의학과
- Issue Date
- 2017-02
- Publisher
- 서울대학교 대학원
- Keywords
- 고양이 줄기세포
- Description
- 학위논문 (석사)-- 서울대학교 대학원 : 수의학과, 2017. 2. 윤화영.
- Abstract
- Cytotherapy with mesenchymal stem cells (MSCs) has been studied in many species, requiring in vitro cell expansion in order to obtain therapeutic doses of stem cells. Since characteristics of MSCs such as self-renewal and multi-lineage differentiation can be altered during long-term culture, it is important to ensure that stemness is maintained during cultivation. This study assessed changes in the characteristics of feline adipose tissue-derived MSCs (fAT-MSCs) during in vitro passaging. Stem cells isolated from the adipose tissue of a donor cat were cultured for seven sub-passages. Proliferation capacity was analyzed by calculating the cell doubling time and by colorimetric assay. Stem cell-specific marker expression was confirmed by quantitative real-time PCR (qRT-PCR) and immunophenotyping. Expression of adipogenic and osteogenic differentiation markers was also confirmed with qRT-PCR. Histochemical staining and measurement of β-galactosidase activity were used to detect cellular senescence. The proliferation rate of the cells was found to decrease significantly at passage 5 (P5). Gene expression levels of pluripotency markers (SOX2, NANOG, and KLF4) and stem cell surface markers (CD9, CD44, CD90, and CD105) all decreased during continuous culture
for most assays, statistically significant reductions were observed at P5. The ability of cells to undergo adipogenic or osteogenic differentiation was inversely proportional to the number of passages. Percentages of senescent cells identified via histochemical staining increased with the number of passages. These results suggest that increasing the number of passages affects the proliferation and multipotency of fAT-MSCs. In clinical trials, early-passage cells should be used for maximum therapeutic effect.
- Language
- Korean
- Files in This Item:
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.