Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis

Fujita, Toshitsugu; Kitaura, Fusako; Oji, Asami; Tanigawa, Naoki; Yuno, Miyuki; Ikawa, Masahito; Taniuchi, Ichiro; Fujii, Hodaka

doi:https://doi.org/10.1111/gtc.12573

アクセス数:123件（2024-05-22 05:55 集計）

固定URL: https://hdl.handle.net/11094/78574

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タイトル	Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis
著者	NRID 1000010550030 1000010550030 著者名 Fujita, Toshitsugu
	著者名 Kitaura, Fusako
	NRID 1000050803417 1000050803417 著者名 Oji, Asami
	著者名 Tanigawa, Naoki
	著者名 Yuno, Miyuki
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000020284573 1000020284573 著者名 Taniuchi, Ichiro
	NRID 1000030302665 1000030302665 著者名 Fujii, Hodaka
キーワード等	ChIP
	chromatin immunoprecipitation
	dCas9
	enChIP
	transgenic mouse
内容	Fujita, T, Kitaura, F, Oji, A, et al. Transgenic mouse lines expressing the 3xFLAG‐dCas9 protein for enChIP analysis. Genes Cells. 2018; 23: 318– 325. https://doi.org/10.1111/gtc.12573
抄録	We developed the engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology to isolate specific genomic regions while retaining their molecular interactions. In enChIP, the locus of interest is tagged with an engineered DNA-binding molecule, such as a modified form of the clustered regularly interspaced short palindromic repeats (CRISPR) system containing a guide RNA (gRNA) and a catalytically inactive form of Cas9 (dCas9). The locus is then affinity-purified to enable identification of associated molecules. In this study, we generated transgenic mice expressing 3xFLAG-tagged Streptococcus pyogenes dCas9 (3xFLAG-dCas9) and retrovirally transduced gRNA into primary CD4+ T cells from these mice for enChIP. Using this approach, we achieved high yields of enChIP at the targeted genomic region. Our novel transgenic mouse lines provide a valuable tool for enChIP analysis in primary mouse cells.
出版者	Blackwell Publishing Ltd
収録物名	Genes to Cells
巻(号)	23(4)
ページ	318-325
刊行年月	2018-04-10
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78574
PISSN	13569597
eISSN	13652443
NCID	AA11623816
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1111/gtc.12573
関連情報 (isIdenticalTo)	PMID 29480524
アクセス権	open access
権利情報	© 2018 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Genes to Cells. 2018, 23(4), p. 318-325
DC.title	Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis
DC.creator	Fujita, Toshitsugu
	Kitaura, Fusako
	Oji, Asami
	Tanigawa, Naoki
	Yuno, Miyuki
	Ikawa, Masahito
	Taniuchi, Ichiro
	Fujii, Hodaka
DC.publisher	Blackwell Publishing Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-04-10
DC.identifier	https://doi.org/10.1111/gtc.12573
DC.subject	ChIP
	chromatin immunoprecipitation
	dCas9
	enChIP
	transgenic mouse
DC.description	Fujita, T, Kitaura, F, Oji, A, et al. Transgenic mouse lines expressing the 3xFLAG‐dCas9 protein for enChIP analysis. Genes Cells. 2018; 23: 318– 325. https://doi.org/10.1111/gtc.12573
DCTERMS.abstract	We developed the engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology to isolate specific genomic regions while retaining their molecular interactions. In enChIP, the locus of interest is tagged with an engineered DNA-binding molecule, such as a modified form of the clustered regularly interspaced short palindromic repeats (CRISPR) system containing a guide RNA (gRNA) and a catalytically inactive form of Cas9 (dCas9). The locus is then affinity-purified to enable identification of associated molecules. In this study, we generated transgenic mice expressing 3xFLAG-tagged Streptococcus pyogenes dCas9 (3xFLAG-dCas9) and retrovirally transduced gRNA into primary CD4+ T cells from these mice for enChIP. Using this approach, we achieved high yields of enChIP at the targeted genomic region. Our novel transgenic mouse lines provide a valuable tool for enChIP analysis in primary mouse cells.
DC.rights	© 2018 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	78574
citation_title	Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis
citation_author	Fujita, Toshitsugu
	Kitaura, Fusako
	Oji, Asami
	Tanigawa, Naoki
	Yuno, Miyuki
	Ikawa, Masahito
	Taniuchi, Ichiro
	Fujii, Hodaka
citation_publisher	Blackwell Publishing Ltd
citation_language	英語
citation_date	2018-04-10
citation_journal_title	Genes to Cells
citation_volume	23
citation_issue	4
citation_firstpage	318
citation_lastpage	325
citation_doi	https://doi.org/10.1111/gtc.12573
citation_pmid	29480524
citation_public_url	https://hdl.handle.net/11094/78574

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