An Investigation into the Biomarker Potential of Highly Branched Isoprenoids in Northern Minnesota Lake Sediments (2022-03-25)
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An Investigation into the Biomarker Potential of Highly Branched Isoprenoids in Northern Minnesota Lake Sediments (2022-03-25)
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2022
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Abstract
Highly Branched Isoprenoids (HBIs) are isoprene-based lipids synthesized
by diatoms. HBIs are often used as sea-ice proxies in marine environments
where certain HBIs indicate the presence of sea ice diatoms. However, few
studies have focused on HBIs produced by freshwater diatoms. The goal
of this study is to expand the knowledge of the types of HBIs produced in
freshwater environments through the identification and quantification of
HBIs in surface sediment and periphyton samples containing HBI-
producing freshwater diatoms collected from small lakes, the St. Louis
River estuary, and Lake Superior. Each of the seven study sites was
sampled twice, once in the summer and once in the winter to determine if
seasonal conditions affect HBI production of diatom communities. At each
site, the entire HBI suite is characterized by gas chromatography-mass
spectrometry (GC-MS) analysis of the nonpolar extractable fraction, and
the genera of diatoms present are characterized by visual identification.
Additionally, single strains of Nitzschia frustulum and Fragilaria capucina
have been isolated and are being grown in liquid media over a range of
temperatures. Eight distinct HBIs have been identified from the seven sites
with concentrations ranging from 0.1-0.9 ?g g-1 dry sediment, only one of
which has been previously identified in the literature. One distinct C30:6
and two distinct C30:4 HBIs have been identified from the single-strain
studies.
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Friday, March 25, 2022, 3:30 p.m.; Chem 200; Bennett Hanson, Master's Student, Department of Chemistry & Biochemistry, University of Minnesota Duluth; Research Advisor: Dr. Kathryn Schreiner
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Hanson, Bennett; University of Minnesota Duluth. Department of Chemistry and Biochemistry. (2022). An Investigation into the Biomarker Potential of Highly Branched Isoprenoids in Northern Minnesota Lake Sediments (2022-03-25). Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/227544.
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