Scale-up studies for stable, long-term indoor and outdoor production of hydrogen by immobilized Rhodobacter capsulatus

Date

2017-09-07

Author

Sagir, EMRAH
ALIPOUR, Siamak
ELKAHLOUT, Kamal
Koku, Harun
Gündüz, Ufuk
Eroglu, Inci
YUCEL, Meral

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

144
views

0
downloads

Photofermentative hydrogen production by immobilized Rhodobacter capsulatus YO3 was carried out in a novel photobioreactor in sequential batch mode under indoor and outdoor conditions. Long-term H-2 production was realized in a 1.4 L photobioreactor for 64 days using Rhodobacter capsulatus YO3 immobilized with 4% (w/v) agar on 5 mM sucrose and 4 mM glutamate. The highest hydrogen yield (19 mol H-2/mol sucrose) and hydrogen productivity (0.73 mmol H-2 L-1 h(-1)) were achieved indoors on 5 mM sucrose. The effect of initial sucrose concentration (5 mM, 10 mM, and 20 mM) on hydrogen production was also investigated. Sustained hydrogen production was carried out under natural, outdoor conditions as well. For the outdoor experiments, the highest hydrogen productivity and yield were obtained as 0.87 +/- 0.06 mmol H-2 L-1 h(-1) and 6.1 +/- 0.2 mol H-2/mol sucrose, respectively on 10 mM sucrose. Furthermore, this system prevented sudden pH drops and fluctuations caused by the utilization of sucrose throughout the process. These results demonstrate that a proper immobilization setup can lead to long-term efficient and robust hydrogen production even under naturally varying conditions. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

Subject Keywords

Biohydrogen, Cell immobilization, Rhodobacter capsulatus, Photobioreactor, Sucrose

URI

https://hdl.handle.net/11511/30338

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2017.07.240

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Biohydrogen production by Rhodobacter capsulatus Hup(-) mutant in pilot solar tubular photobioreactor Boran, Efe; Ozgur, Ebru; Yucel, Meral; Gündüz, Ufuk; EROĞLU, İNCİ (2012-11-01) In this study, a pilot solar tubular photobioreactor was successfully implemented for fed batch operation in outdoor conditions for photofermentative hydrogen production with Rhodobacter capsulatus (Hup(-)) mutant. The bacteria had a rapid growth with a specific growth rate of 0.052 h(-1) in the batch exponential phase and cell dry weight remained in the range of 1-1.5 g/L throughout the fed batch operation. The feeding strategy was to keep acetic acid concentration in the photobioreactor at the range of 20...
Biohydrogen production by Rhodobacter capsulatus in solar tubular photobioreactor on thick juice dark fermenter effluent Boran, Efe; Ozgur, Ebru; Yucel, Meral; Gündüz, Ufuk; Eroglu, Inci (2012-08-01) The purpose of this study was to operate a pilot scale solar tubular photobioreactor (90 L) for photofermentative hydrogen production from thick juice dark fermenter effluent (DEE) by photosynthetic purple-non-sulfur bacterium, Rhodobacter capsulatus. The tubular photobioreactor was equipped with internal cooling tubes for effective temperature control, which is essential for stable, long-term operation. The photobioreactor was operated in fed-batch mode throughout September 2009 in Ankara, Turkey. R. capsu...
Implementation and analysis of temperature control strategies for outdoor photobiological hydrogen production ANDROGA, DOMINIC DEO; UYAR, BAŞAR; Koku, Harun; EROĞLU, İNCİ (2016-12-01) For outdoor photobiological hydrogen production, the effective control of temperature in photobioreactors is a challenge. In this work, an internal cooling system for outdoor tubular photobioreactors was designed, built, and tested. The temperatures in the reactors with bacteria were consistently higher than those without bacteria, and were also strongly influenced by solar irradiation and ambient air temperature. The cooling protocol applied successfully kept the reactor temperatures below the threshold li...
Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice in outdoor conditions Ozkan, Endam; Uyar, Basar; Ozgur, Ebru; Yucel, Meral; Eroglu, Inci; Gündüz, Ufuk (2012-01-01) In the present study, photofermentative hydrogen production on thermophilic dark fermentation effluent (DFE) of sugar beet thick juice was investigated in a solar fed-batch panel photobioreactor (PBR) using Rhodobacter capsulatus YO3 (hup(-)) during summer 2009 in Ankara, Turkey. The DFE was obtained by continuous dark fermentation of sugar beet thick juice by extreme thermophile Caldicellulosiruptor saccharolyticus and it contains acetate (125 mM) and NH4+ (7.7 mM) as the main carbon and nitrogen sources, ...
Continuous hydrogen production by Rhodobacter sphaeroides O.U.001 Eroğlu, İnci; Aslan, K; Gunduz, M; Yucel, M; Turker, L (1997-06-26) This paper describes hydrogen gas production by Rhodobacter sphaeroides O.U.001 using a column photobioreactor in batch and continuous operation. The effect of substrates on the hydrogen production rate was investigated in batch-type photobioreactor experiments. Substrate concentrations (L-malic acid and sodium glutamate) were measured by using high-pressure liquid chromatography. The gas produced was analyzed by gas chromatography.

Citation Formats

E. Sagir et al., “Scale-up studies for stable, long-term indoor and outdoor production of hydrogen by immobilized Rhodobacter capsulatus,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 22743–22755, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30338.