The preprotein translocation channel of the outer membrane of mitochondria

Kunkele, K. P.; Heins, S.; Dembowski, M.; Nargang, F. E.; Benz, R.; Thieffry, M.; Walz, J.; Lill, R.; Nussberger, S.; Neupert, W.

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The preprotein translocation channel of the outer membrane of mitochondria

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Kunkele, K. P., Heins, S., Dembowski, M., Nargang, F. E., Benz, R., Thieffry, M., Walz, J., Lill, R., Nussberger, S., & Neupert, W. (1998). The preprotein translocation channel of the outer membrane of mitochondria. Cell, 93(6), 1009-1019.

引用: https://hdl.handle.net/11858/00-001M-0000-0010-7209-A

要旨

The preprotein translocase of the outer membrane of mitochondria (TOM complex) facilitates the recognition, insertion, and translocation of nuclear-encoded mitochondrial preproteins. We have purified the TOM complex from Neurospora crassa and analyzed its composition and functional properties. The TOM complex contains a cation-selective high-conductance channel. Upon reconstitution into liposomes, it mediates integration of proteins into and translocation across the lipid bilayer. TOM complex particles have a diameter of about 138 Angstrom, as revealed by electron microscopy and image analysis; they contain two or three centers of stain-filled openings, which we interpret as pores with an apparent diameter of about 20 Angstrom. We conclude that the structure reported here represents the protein-conducting channel of the mitochondrial outer membrane. [References: 75]