FRET Imaging

Jares-Erijman, E. A.; Jovin, T. M.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

FRET Imaging

MPS-Authors

/persons/resource/persons15041

Jares-Erijman, E. A.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15286

Jovin, T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Jares-Erijman, E. A., & Jovin, T. M. (2003). FRET Imaging. Nature biotechnology, 21(11), 1387-1395. Retrieved from http://www.nature.com/nbt/journal/v21/n11/pdf/nbt896.pdf.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-EFE0-8

Abstract

Förster (or Fluorescence) Resonance Energy Transfer (FRET) generates fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a new photophysical framework for the phenomenon and apply it to the wide range of FRET techniques adapted to imaging systems. A systematic catalogue of 21 such methods is presented, including several new approaches proposed as suitable prospects for implementation. We conclude with a brief discussion of selected probe issues and anticipated future developments extending from single molecule to live cell applications.