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Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL)

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Maccarrone,  Giuseppina
Dept. Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Max Planck Society;

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Lebar,  Maria
Max Planck Institute of Psychiatry, Max Planck Society;

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Martins-de-Souza,  Daniel
Max Planck Institute of Psychiatry, Max Planck Society;

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Citation

Maccarrone, G., Lebar, M., & Martins-de-Souza, D. (2014). Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL).


Cite as: https://hdl.handle.net/11858/00-001M-0000-0026-B6C3-3
Abstract
Proteomics has been revolutionized by the rapid advance of mass spectrometric instrumentations and techniques. Parallel methodologies for the quantification of proteomes also evolved, including in vitro stable isotope labeling. Here, we present a protocol for employing isotope-coded protein labeling (ICPL) as part of a shotgun proteomics workflow denoting its advantages and disadvantages. This protocol is suitable to studying any proteome of interest, only requiring a specific sample preparation and protein identification. Given our expertise, descriptions here are centered on the study of brain disorders.