Cellular resolution circuit mapping with temporal-focused excitation of 
soma-targeted channelrhodopsin

Baker, Christopher A.; Elyada, Yishai M.; Parra, Andres; Bolton, M. McLean

doi:10.7554/eLife.14193

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Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin

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Baker, Christopher A.
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Elyada, Yishai M.
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Parra, Andres
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Bolton, M. McLean
Max Planck Florida Institute for Neuroscience, Max Planck Society;

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Citation

Baker, C. A., Elyada, Y. M., Parra, A., & Bolton, M. M. (2016). Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin. eLife, 5, e14193-e14193. doi:10.7554/eLife.14193.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-2ADA-9

Abstract

We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.