Expansion microscopy: principles and uses in biological research
Author(s)
Wassie, Asmamaw T.; Zhao, Yongxin; Boyden, Edward
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Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial resolution. We recently discovered that preserved biological specimens can be physically expanded in an isotropic fashion through a chemical process. Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout chemistries, and makes specimens transparent. We review the principles of how ExM works, advances in the technology made by our group and others, and its applications throughout biology and medicine.
Date issued
2018-12Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; Massachusetts Institute of Technology. Department of Biological Engineering; McGovern Institute for Brain Research at MIT; Massachusetts Institute of Technology. Media Laboratory; Massachusetts Institute of Technology. Center for Neurobiological Engineering; Koch Institute for Integrative Cancer Research at MITJournal
Nature Methods
Publisher
Springer Nature
Citation
Wassie, Asmamaw T. et al., "Expansion Microscopy: Principles and Uses in Biological Research." Nature Methods 16, 1 (January 2019): 33–41 @2018 Authors
Version: Author's final manuscript
ISSN
1548-7105