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https://hdl.handle.net/1822/34735
Título: | Enzymatic synthesis of poly(catechin)-antibiotic conjugates: an antimicrobial approach for indwelling catheters |
Autor(es): | Gonçalves, Idalina Abreu, Ana S. Matamá, Maria Teresa Ribeiro, Artur J. Gomes, Andreia C Silva, Carla Manuela Pereira Marinho da Paulo, Artur Cavaco |
Palavras-chave: | Laccase Catechin Trimethoprim Sulfamethoxazole Antimicrobial Catheters |
Data: | 2015 |
Editora: | Springer |
Revista: | Applied Microbiology and Biotechnology |
Resumo(s): | Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N′-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing α-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase oxidation of catechin-antibiotic conjugates. Four antimicrobial coatings were produced, namely with poly(catechin), poly(catechin)- TMP, poly(catechin)-SMZ and poly(catechin)-TMP-SMZ. The bacterial adhesion reduction was tested on the functionalized devices using gram-negative and gram-positive strains. The most significant reduction in adhesion was observed with poly(catechin)-TMP (gram-negative −85 % and grampositive −87 %) and with poly(catechin)-TMP-SMZ (gramnegative −85 % and gram-positive −91 %). The cytotoxicity to mammalian cells was tested by indirect contact for 5 days and revealed that all the tested coatings supported more than 90 % of viable cells. A promising approach for the increase of the indwelling catheter lifespan was developed aiming to reduce catheter-associated chronic infections. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/34735 |
DOI: | 10.1007/s00253-014-6128-2 |
ISSN: | 0175-7598 1432-0614 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers DBio - Artigos/Papers IPC - Artigos em revistas científicas internacionais com arbitragem |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Enzymatic synthesis of poly(catechin)-antibiotic conjugates an antimicrobial approach for indwelling catheters.pdf | 1,86 MB | Adobe PDF | Ver/Abrir |