Advancing cancer detection: Multi-photon microscopy setup for NADH and FAD fluorescence analysis

Abstract

Multi-photon microscopy (MPM) revolutionizes cancer diagnosis and can replace histopathology during colonoscopy. Based on endogenous fluorophores such as NADH and FAD, MPM offers high sensitivity. This technique enables real-time imaging and optical sectioning, potentially enhancing early-stage colon cancer detection [1,2]. The focus of this work is on the implementation of an MPM setup for NADH and FAD detection. A future integration of a MPM probe in conventional colonoscopy may offers in vivo optical biopsy, advancing cancer monitoring and diagnosis in early-stage cancer detection.