Leiden University Scholarly Publications

Crone, N.S.A. (2021)

Synthetic modification of fusogenic coiled coil peptides

Doctoral Thesis

A structural investigation of coiled coil peptides used as membrane fusogens, mimicing naturally occuring coiled-coil fusion proteins. Synthetic modifications have been made to alter lipid attachment, secondary structure and to insert photoactive azobenzene moieties for active control over coiled coil structure. Finally, the underlying photocontrol mechanism investigated in coiled coil peptides is extended to beta-structured peptides, and was shown to be universally applicable.

Peptides

Coiled coils

Membrane fusion

Synthetic biology

Liposomes

Peptide stapling

Azobenzenes

Photoswitching

Synthetic chemistry

All authors

Crone, N.S.A.

Supervisor

Kros, A.

Co-supervisor

Boyle, A.L.

Committee

Overkleeft, H.S.; Bonnet, S.A.; Wezenberg, S.J.; Ashkenasy, G.; Thomas, F.

Qualification

Doctor (dr.)

Awarding Institution

Leiden Institute of Chemistry (LIC), Faculty of Science, Leiden University

Date

2021-12-16

ISBN (print)

9789464215687