Microbial enzyme activities: peptidase activities in bulk seawater samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012

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Date Created
2018-07-24
Location
Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
westlimit: 31.21; southlimit: 79.6502; eastlimit: 130.5795; northlimit: 88.809
DOI
10.26008/1912/bco-dmo.742780.1
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Abstract
This dataset includes peptidase activities measured in bulk (not filter-fractionated) seawater. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/742780
Description
Dataset: ARK27-3: Bulk MCA hydrolysis rates
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Citation
Arnosti, C. (2020). Microbial enzyme activities: peptidase activities in bulk seawater samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012 (Version 1) [Data set]. Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://doi.org/10.26008/1912/BCO-DMO.742780.1
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