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TITLE:
Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium - imarina:9292253

URV's Author/s:Constantí Garriga, Magdalena / GUNASEKARAN, VIJAYALAKSHMI
Author, as appears in the article.:Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda;
Author's mail:magdalena.constanti@urv.cat
Author identifier:0000-0002-3547-4199
Journal publication year:2022
Publication Type:Journal Publications
APA:Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda; (2022). Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium. Microorganisms, 10(12), -. DOI: 10.3390/microorganisms10122331
Papper original source:Microorganisms. 10 (12):
Abstract:A bacterial consortium capable of degrading ethyl tert-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. Arthrobacter sp., Herbaspirillum sp., Pseudacidovorax sp., Pseudomonas sp., and Xanthomonas sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). Xanthomonas sp. and Pseudomonas sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community.
Article's DOI:10.3390/microorganisms10122331
Link to the original source:https://www.mdpi.com/2076-2607/10/12/2331
Papper version:info:eu-repo/semantics/publishedVersion
licence for use:https://creativecommons.org/licenses/by/3.0/es/
Department:Enginyeria Química
Licence document URL:https://repositori.urv.cat/ca/proteccio-de-dades/
Thematic Areas:Virology
Microbiology (medical)
Microbiology
Keywords:Strain
Sp nov.
Pathway
Mtbe
Metaproteome
Inhalation exposure
Hydrocarbons
Herbaspirillum-chlorophenolicum
Etbe
Degradation
Biodegradation
Bacterial consortium
2d-dige
Entity:Universitat Rovira i Virgili
Record's date:2024-09-07
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