[en] Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteraceae family, genome analyses revealed that bacteria that possess an Acx, such as Cupriavidus metallidurans strain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α(2)β(2)γ(2) and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.
Disciplines :
Zoology
Author, co-author :
Rosier, Caroline ; Université de Mons - UMONS > Faculté des Sciences > Protéomie et Microbiologie
Leys, Natalie; Expert Group for Molecular and Cellular Biology, Mol, Belgium
Henoumont, Céline ; Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, Mons, Belgium
Mergeay, Maximilien ; Université de Mons - UMONS > Faculté des Sciences > Protéomie et Microbiologie ; Expert Group for Molecular and Cellular Biology, Mol, Belgium
Wattiez, Ruddy ; Université de Mons - UMONS > Faculté des Sciences > Service de Protéomie et Microbiologie
Language :
English
Title :
Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34.
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