Molecular counting of membrane receptor subunits with single-molecule 
localization microscopy

Krüger, Carmen; Fricke, Franziska; Karathanasis, Christos; Dietz, Marina S.; Malkusch, Sebastian; Hummer, Gerhard; Heilemann, Mike

doi:10.1117/12.2256638

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Molecular counting of membrane receptor subunits with single-molecule localization microscopy

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Hummer, Gerhard
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;
Institute of Biophysics, Goethe-University Frankfurt;

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Citation

Krüger, C., Fricke, F., Karathanasis, C., Dietz, M. S., Malkusch, S., Hummer, G., et al. (2017). Molecular counting of membrane receptor subunits with single-molecule localization microscopy. In Proceedings SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X. doi:10.1117/12.2256638.

Cite as: https://hdl.handle.net/21.11116/0000-0001-28E5-0

Abstract

We report on quantitative single-molecule localization microscopy, a method that next to super-resolved images of cellular structures provides information on protein copy numbers in protein clusters. This approach is based on the analysis of blinking cycles of single fluorophores, and on a model-free description of the distribution of the number of blinking events. We describe the experimental and analytical procedures, present cellular data of plasma membrane proteins and discuss the applicability of this method