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Magneto Immuno-MR: A novel immunoassay based on biogenic magnetosome nanoparticles

MPG-Autoren
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Schüler,  D.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Lang,  C.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Zitation

Wacker, R., Ceyhan, B., Alhorn, P., Schüler, D., Lang, C., & Niemeyer, C. M. (2007). Magneto Immuno-MR: A novel immunoassay based on biogenic magnetosome nanoparticles. Biochemical and Biophysical Research Communications, 357(2), 391-396.


Zitierlink: https://hdl.handle.net/21.11116/0000-0001-CE4C-3
Zusammenfassung
We describe an innovative modification of the Immuno-PCR technology for automatable high sensitive antigen detection. The Magneto Immuno-PCR (M-IPCR) is based on antibody-functionalized biogenic magnetosome nanoparticles revealing major advantages over synthetic magnetic particles. The general principle of the M-IPCR is similar to that of a two-sided (sandwich) immunoassay. However, antibody-functionalized magnetosome conjugates were employed for the immobilization and magnetic enrichment of the signal generating detection complex enabling the establishment of a surface independent immunoassay. To this end, the M-IPCR was carried out by simultaneously tagging the antigen with the reagent for read-out, i.e., a conjugate comprising the specific antibody and DNA fragments, in the presence of the antibody-functionalized magnetosomes. To demonstrate the general functionality of the M-IPCR, the detection of recombinant Hepatitis B surface Antigen (HBsAg) in human serum was established. We observed a detection limit of 320 pg/ml of HBsAg using the M-IPCR, which was about 100-fold more sensitive than the analogous Magneto-ELISA, established in parallel for comparison purposes.