Core region of Citrobacter lipopolysaccharide from strain PCM 1487 Structure 
elucidation by two‐dimensional 1H‐NMR spectroscopy at 500 MHz and methylation 
analysis/mass spectrometry

Romanowska , Elzbieta; Gamian, Andrej; Dabrowski, Janusz

doi:10.1111/j.1432-1033.1986.tb10478.x

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Core region of Citrobacter lipopolysaccharide from strain PCM 1487 Structure elucidation by two‐dimensional 1H‐NMR spectroscopy at 500 MHz and methylation analysis/mass spectrometry

MPS-Authors

/persons/resource/persons92575

Dabrowski, Janusz
Department of Organic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://febs.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1432-1033.1986.tb10478.x
(Any fulltext)

https://doi.org/10.1111/j.1432-1033.1986.tb10478.x
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Romanowska, E., Gamian, A., & Dabrowski, J. (1986). Core region of Citrobacter lipopolysaccharide from strain PCM 1487 Structure elucidation by two‐dimensional 1H‐NMR spectroscopy at 500 MHz and methylation analysis/mass spectrometry. European Journal of Biochemistry, 161(3), 557-564. doi:10.1111/j.1432-1033.1986.tb10478.x.

Cite as: https://hdl.handle.net/21.11116/0000-0006-9422-C

Abstract

The core structure of Citrobacter PCM 1487 lipopolysaccharide has been established using methylation analysis/mass spectrometry, chemical degradations and one- and two-dimensional 1H-NMR spectroscopy at 500 MHz. 1H-NMR assignments are given for all sugar components of the core oligosaccharide. In the formula shown below, the alternative locations of branch terminal heptose (LDHep) and diphosphorylethanolamine (PPEtN) residues are marked by dashed lines; dOclA stands for 3-deoxy-D-manno-octulosonic acid. (Formula: see text). The sample of the core oligosaccharide showed some microheterogeneity due to a slightly incomplete substitution by terminal N-acetylgalactosamine and a partial splitting of diphosphorylethanolamine residues.