Molecular counting of membrane receptor subunits with single-molecule 
localization microscopy

Krüger, Carmen; Fricke, Franziska; Karathanasis, Christos; Dietz, Marina S.; Malkusch, Sebastian; Hummer, Gerhard; Heilemann, Mike

doi:10.1117/12.2256638

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

会議論文

Molecular counting of membrane receptor subunits with single-molecule localization microscopy

MPS-Authors

/persons/resource/persons15259

Hummer, Gerhard
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;
Institute of Biophysics, Goethe-University Frankfurt;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Krüger, C., Fricke, F., Karathanasis, C., Dietz, M. S., Malkusch, S., Hummer, G., & Heilemann, M. (2017). Molecular counting of membrane receptor subunits with single-molecule localization microscopy. In Proceedings SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X. doi:10.1117/12.2256638.

引用: https://hdl.handle.net/21.11116/0000-0001-28E5-0

要旨

We report on quantitative single-molecule localization microscopy, a method that next to super-resolved images of cellular structures provides information on protein copy numbers in protein clusters. This approach is based on the analysis of blinking cycles of single fluorophores, and on a model-free description of the distribution of the number of blinking events. We describe the experimental and analytical procedures, present cellular data of plasma membrane proteins and discuss the applicability of this method