Using cryo-EM to understand the assembly pathway of respiratory complex I

Laube, Eike; Schiller, Jonathan; Zickermann, Volker; Vonck, Janet

doi:10.1107/S205979832400086X

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Using cryo-EM to understand the assembly pathway of respiratory complex I

MPS-Authors

/persons/resource/persons283055

Laube, Eike
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons137933

Vonck, Janet
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

laubevonck.pdf
(全文テキスト（全般）), 8MB

付随資料 (公開)

There is no public supplementary material available

引用

Laube, E., Schiller, J., Zickermann, V., & Vonck, J. (2024). Using cryo-EM to understand the assembly pathway of respiratory complex I. Acta Crystallographica Section D: Structural Biology. doi:10.1107/S205979832400086X.

引用: https://hdl.handle.net/21.11116/0000-000E-70E1-5

要旨

Complex I (proton-pumping NADH:ubiquinone oxidoreductase) is the first component of the mitochondrial respiratory chain. In recent years, high-resolution cryo-EM studies of complex I from various species have greatly enhanced the understanding of the structure and function of this important membrane-protein complex. Less well studied is the structural basis of complex I biogenesis. The assembly of this complex of more than 40 subunits, encoded by nuclear or mitochondrial DNA, is an intricate process that requires at least 20 different assembly factors in humans. These are proteins that are transiently associated with building blocks of the complex and are involved in the assembly process, but are not part of mature complex I. Although the assembly pathways have been studied extensively, there is limited information on the structure and molecular function of the assembly factors. Here, the insights that have been gained into the assembly process using cryo-EM are reviewed.