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Relationship between the acceptor/donor radioactivity ratio and cross-linking in bacterial peptidoglycan: application to surface synthesis during the division cycle

MPG-Autoren
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Cooper,  S
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Zitation

Cooper, S. (1990). Relationship between the acceptor/donor radioactivity ratio and cross-linking in bacterial peptidoglycan: application to surface synthesis during the division cycle. Journal of Bacteriology, 172(9), 5506-5510. doi:10.1128/jb.172.9.5506-5510.1990.


Zitierlink: https://hdl.handle.net/21.11116/0000-000F-2118-1
Zusammenfassung
The relationship between the experimental measurement of the cross-linking of bacterial peptidoglycan and the mode of its insertion is analyzed. The cross-linking value, in practice and in theory, is independent of the pattern of strand insertion. Since the measure of the mode or pattern of insertion is the acceptor/donor radioactivity ratio (ADRR), no correction need be made for the ADRR. The measurement of cross-linking using radioactivity is independent of the labeling time, the specific activity of the label, and the mode of strand insertion. It is not concluded, however, that cross-linking does increase during the division cycle.