Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess
Аутори
Santrač, IsidoraDanilović Luković, Jelena
Dimitrijević, Milena
Stanić, Marina
Tanović, Marija
Ćurić, Valentina
Kovačević, Snežana
Zechmann, Bernd
Žižić, Milan
Spasojević, Ivan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Haematococcus pluvialis is a unicellular green alga with a complex life cycle and a
remarkable metabolic and structural adaptability that allows it to thrive in metal-infested
environments. H. pluvialis could be potentially used in the remediation of waters polluted
with metals, such as manganese (Mn). Mn is also an essential element involved in different
metabolic processes, such as photosynthesis and antioxidantive defence. Herein, we
examined morphological response of metabolically active green cell type of H. pluvialis
(microzooids) to high Mn concentrations exceeding their physiological quota. When exposed
to 1 mM Mn2+, cell viability remained stable over a 3-day period. Inductively coupled plasma
atomic emission spectroscopy showed the prompt uptake of Mn by the microzooid cells after
1 h of the treatment, with a modest increase of the concentration of Mn in the biomass at 24
h. Scanning electron microscopy revealed granular deposits on microzooid surfaces after 1
hour..., likely Mn deposits, while transmission electron microscopy (TEM) micrographs
showed that some cells had wall rupture and degraded intracellular content and damaged
organelles. After 24 and 72 h, a different type of cell morphology emerged, characterized by
thickened cell wall, preserved intracellular compartments, and reduced total area of lipid
droplets. Both cell types exhibited vacuoles containing dark granules, possibly indicative of
Mn accumulations. Quantitative TEM analysis demonstrated that an excess of Mn reduced
cell cross-section and lipid droplet area while increasing vacuole cross-section and cell wall
thickness. The intricate adaptive responses of H. pluvialis to elevated Mn concentrations
exemplified by cell wall thickening, reduction in lipid droplets total area due to increased
energy demand, and the accumulation of Mn in vacuoles, exhibits the impressive structural
adaptability. Further investigation using analytical methods will provide a more profound
understanding of the metabolic dimensions of adaptive response
Кључне речи:
microalgae / Haematococcus pluvialis / manganese / bioremediation / ICP / electron microscopyИзвор:
International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia, 2023, 44-44Издавач:
- UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Santrač, Isidora AU - Danilović Luković, Jelena AU - Dimitrijević, Milena AU - Stanić, Marina AU - Tanović, Marija AU - Ćurić, Valentina AU - Kovačević, Snežana AU - Zechmann, Bernd AU - Žižić, Milan AU - Spasojević, Ivan PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3144 AB - Haematococcus pluvialis is a unicellular green alga with a complex life cycle and a remarkable metabolic and structural adaptability that allows it to thrive in metal-infested environments. H. pluvialis could be potentially used in the remediation of waters polluted with metals, such as manganese (Mn). Mn is also an essential element involved in different metabolic processes, such as photosynthesis and antioxidantive defence. Herein, we examined morphological response of metabolically active green cell type of H. pluvialis (microzooids) to high Mn concentrations exceeding their physiological quota. When exposed to 1 mM Mn2+, cell viability remained stable over a 3-day period. Inductively coupled plasma atomic emission spectroscopy showed the prompt uptake of Mn by the microzooid cells after 1 h of the treatment, with a modest increase of the concentration of Mn in the biomass at 24 h. Scanning electron microscopy revealed granular deposits on microzooid surfaces after 1 hour, likely Mn deposits, while transmission electron microscopy (TEM) micrographs showed that some cells had wall rupture and degraded intracellular content and damaged organelles. After 24 and 72 h, a different type of cell morphology emerged, characterized by thickened cell wall, preserved intracellular compartments, and reduced total area of lipid droplets. Both cell types exhibited vacuoles containing dark granules, possibly indicative of Mn accumulations. Quantitative TEM analysis demonstrated that an excess of Mn reduced cell cross-section and lipid droplet area while increasing vacuole cross-section and cell wall thickness. The intricate adaptive responses of H. pluvialis to elevated Mn concentrations exemplified by cell wall thickening, reduction in lipid droplets total area due to increased energy demand, and the accumulation of Mn in vacuoles, exhibits the impressive structural adaptability. Further investigation using analytical methods will provide a more profound understanding of the metabolic dimensions of adaptive response PB - UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade C3 - International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia T1 - Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess EP - 44 SP - 44 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3144 ER -
@conference{ author = "Santrač, Isidora and Danilović Luković, Jelena and Dimitrijević, Milena and Stanić, Marina and Tanović, Marija and Ćurić, Valentina and Kovačević, Snežana and Zechmann, Bernd and Žižić, Milan and Spasojević, Ivan", year = "2023", abstract = "Haematococcus pluvialis is a unicellular green alga with a complex life cycle and a remarkable metabolic and structural adaptability that allows it to thrive in metal-infested environments. H. pluvialis could be potentially used in the remediation of waters polluted with metals, such as manganese (Mn). Mn is also an essential element involved in different metabolic processes, such as photosynthesis and antioxidantive defence. Herein, we examined morphological response of metabolically active green cell type of H. pluvialis (microzooids) to high Mn concentrations exceeding their physiological quota. When exposed to 1 mM Mn2+, cell viability remained stable over a 3-day period. Inductively coupled plasma atomic emission spectroscopy showed the prompt uptake of Mn by the microzooid cells after 1 h of the treatment, with a modest increase of the concentration of Mn in the biomass at 24 h. Scanning electron microscopy revealed granular deposits on microzooid surfaces after 1 hour, likely Mn deposits, while transmission electron microscopy (TEM) micrographs showed that some cells had wall rupture and degraded intracellular content and damaged organelles. After 24 and 72 h, a different type of cell morphology emerged, characterized by thickened cell wall, preserved intracellular compartments, and reduced total area of lipid droplets. Both cell types exhibited vacuoles containing dark granules, possibly indicative of Mn accumulations. Quantitative TEM analysis demonstrated that an excess of Mn reduced cell cross-section and lipid droplet area while increasing vacuole cross-section and cell wall thickness. The intricate adaptive responses of H. pluvialis to elevated Mn concentrations exemplified by cell wall thickening, reduction in lipid droplets total area due to increased energy demand, and the accumulation of Mn in vacuoles, exhibits the impressive structural adaptability. Further investigation using analytical methods will provide a more profound understanding of the metabolic dimensions of adaptive response", publisher = "UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade", journal = "International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia", title = "Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess", pages = "44-44", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3144" }
Santrač, I., Danilović Luković, J., Dimitrijević, M., Stanić, M., Tanović, M., Ćurić, V., Kovačević, S., Zechmann, B., Žižić, M.,& Spasojević, I.. (2023). Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade., 44-44. https://hdl.handle.net/21.15107/rcub_rimsi_3144
Santrač I, Danilović Luković J, Dimitrijević M, Stanić M, Tanović M, Ćurić V, Kovačević S, Zechmann B, Žižić M, Spasojević I. Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia. 2023;:44-44. https://hdl.handle.net/21.15107/rcub_rimsi_3144 .
Santrač, Isidora, Danilović Luković, Jelena, Dimitrijević, Milena, Stanić, Marina, Tanović, Marija, Ćurić, Valentina, Kovačević, Snežana, Zechmann, Bernd, Žižić, Milan, Spasojević, Ivan, "Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess" in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia (2023):44-44, https://hdl.handle.net/21.15107/rcub_rimsi_3144 .