Habituation of plant cells does not mean insensitivity to plant growth regulators

[en] Fully habituated organogenic and nonorganogenic sugarbeet calluses reacted to application of the synthetic auxin [3-benzo(b) selenienyl] acetic acid by changes in growth and ethylene production. Treatment of fully habituated cells of periwinkle with 2,4-dichlorophenoxyacetic acid led to the decrease of free cytokinin contents (isopentenyl adenine, zeatin riboside, and zeatin) during the late exponential phase of growth. The polyamine contents were also modified and the capacity to biotransform secologanin into ajmalicine was decreased. Treatment of the habituated periwinkle cells with zeatin greatly increased the amount of a polypeptide of 16 kDa; this response was more marked than that displayed by the auxin-dependent line. These data show that hormone-independent calluses and cell suspensions can retain some sensitivity to growth hormones. However, differences of responses were observed between the auxin-dependent lines and the habituated lines. © 1996 Society for In Vitro Biology.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Kevers, Claire ; Université de Liège > Département des sciences de la vie > Département des sciences de la vie

Filali, M.; Laboratoire de Biologie Cellulaire et Biochimie Végétale EA 1370, Université de Tours, Faculté de Pharmacie, 31 avenue Monge, Tours, 37200, France

Petit-Paly, G.; Laboratoire de Biologie Cellulaire et Biochimie Végétale EA 1370, Université de Tours, Faculté de Pharmacie, 31 avenue Monge, Tours, 37200, France

Hagège, D.; Laboratoire de Biochimie et Physiologie Végétale, Université de Bretagne Occidentale, BP 809, Brest, 29275, France

Rideau, M.; Laboratoire de Biologie Cellulaire et Biochimie Végétale EA 1370, Université de Tours, Faculté de Pharmacie, 31 avenue Monge, Tours, 37200, France

Gaspar, Th; Hormonologie fondamentale et appliquée, Institut de Botanique B-22, Université de Liège, Sart Tilman, Liège, B-4000, Belgium

Language :

English

Title :

Habituation of plant cells does not mean insensitivity to plant growth regulators

Publication date :

1996

Journal title :

In Vitro Cellular and Developmental Biology. Plant

ISSN :

1054-5476

eISSN :

1475-2689

Publisher :

Springer, Heidelberg, Germany

Volume :

Issue :

Pages :

204-209

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 15 July 2015

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