[en] Late embryonic and early fetal mortality (EM/FM) might occur following a pregnancy diagnosis, decreasing the accuracy of pregnancy diagnostics in dairy cattle. Therefore, the detection of EM/FM may be useful in dairy reproductive management. Comparatively low concentrations of pregnancy-associated glycoproteins (PAGs) have been previously associated with EM/FM. The aim of the present study was to test the ability of a semi- quantitative enzyme-linked immunosorbent assay for the detection of PAGs(PAG-ELISA) to either diagnose (EM/FM has already occurred) or to predict (EM/FM will occur) EM/FM. Three experiments were carried out as follows: (1) PAG-ELISA validation, (2) aretro-spective verification of 141 PAG results in cases with suspected EM/FM and the identification of cut-off values for EM/FM, and (3) a field experiment, testing the proposed PAG-ELISA cut-off values defined in Experiment 2 under farm conditions by comparing transrectal ultrasound (TRU) results with PAG-ELISA results. The PAG-ELISA validation (Experiment 1) demonstrated that anoptical density (OD) range of 0.4–2.8 reflected a linear relation to the PAG concentrations. The results from Experiment 2 indicated that more cows (85.7%) with PAG-OD values ranging from 0.40–0.79 showed EM/FM compared to cows (21.7%) with PAG-OD values ranging from 1.2 to 1.5. Acut-off valueof 2.2 (sensitivity [SEN]=87.6%; specificity [SPE]=72.8%) was determined using ROC-curve analysis for prediction of EM/FM and a cut-off value of 1.2 for diagnosis of EM/FM (SEN=50%; SPE=94%). However, in the field experiment, EM/FM was best diagnosed using a cut-off value of 1.5 (SEN=26.7% and SPE=96.5%). Hence, the high SEN detected in Experiment 2 could not be confirmed in the field experiment indicating that a singular PAG value is not a convenient tool to diagnose EM/FM. Though, the combined use of PAG-ELISA and TRU showed remarkable results (SEN=84.2%; SPE=98.1%) by revealing a technique for diagnosis of previously occurred EM/FM and for prediction of subsequent occurring EM/FM.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Engelke, Janna; University of Veterinary Medicine Hannover, Germany > Clinic for Cattle, Endocrinology Laboratory, Bischofsholer Damm15, 30173 Hannover,
Knaack, Hendrike; University of Veterinary Medicine Hannover, Germany > Clinic for Cattle, Endocrinology Laboratory, Bischofsholer Damm 15, 30173 Hannover
Linden, Matthias; Leibniz University Hannover, Germany > Faculty of Mathematics and Physics, Welfengarten 1, 30167 Hannover
Feldmann, Maren; University of Veterinary Medicine Hannover, Germany > Clinic for Cattle, Bischofsholer Damm 15, 30173 Hannover
Gundling, Natascha; University of Veterinary Medicine Hannover, Germany > Clinic for Cattle, Bischofsholer Damm 15, 30173 Hannover
Gundelach, Yasmin; University of Veterinary Medicine Hannover, Germany > Clinic for Cattle, Bischofsholer Damm 15, 30173 Hannover
Greiner, Matthias; University of Veterinary Medicine Hannover, Germany > Institute for Food Quality and Food Safety, Bischofsholer Damm 15, 30173 Hannover
Beckers, Jean-François ; Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Melo de Sousa, Noelita ; Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Meyerholz, Marie Margarete; University of Veterinary Medicine Hannover, Germany > Clinic for Cattle, Endocrinology Laboratory, Bischofsholer Damm 15, 30173 Hannover
Hoedemaker, Martina; Leibniz University Hannover, Germany > Faculty of Mathematics and Physics, Welfengarten 1, 30167 Hannover
Piechotta, Marion; University of Veterinary Medicine Hannover, Germany > Clinic for Cattle, Endocrinology Laboratory, Bischofsholer Damm 15, 30173 Hannover
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